Stem Cells and Regenerative Medicine Centre, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore, Karnataka, India.
J Cell Biochem. 2020 Jun;121(5-6):3058-3069. doi: 10.1002/jcb.29571. Epub 2019 Dec 30.
The transmembrane phosphoglycoprotein protein CD34 has conventionally been regarded as a marker for hematopoietic progenitors. Its expression on these cells has been leveraged for cell therapy applications in various hematological disorders. More recently, the expression of CD34 has also been reported on cells of nonhematopoietic origin. The list includes somatic cells such as endothelial cells, fibrocytes and interstitial cells and regenerative stem cells such as corneal keratocytes, muscle satellite cells, and muscle-derived stem cells. Furthermore, its expression on some cancer stem cells (CSCs) has also been reported. Till date, the functional roles of this molecule have been implicated in a multitude of cellular processes including cell adhesion, signal transduction, and maintenance of progenitor phenotype. However, the complete understanding about this molecule including its developmental origins, its embryonic connection, and associated functions is far from complete. Here, we review our present understanding of the structure and putative functions of the CD34 molecule based upon our literature survey. We also probed various biological databases to retrieve data related to the expression and associated molecular functions of CD34. Such information, upon synthesis, is hence likely to provide the suitability of such cells for cell therapy. Moreover, we have also covered the existing cell therapy and speculated cell therapy applications of CD34 cells isolated from various lineages. We have also attempted here to speculate the role(s) of CD34 on CSCs. Finally, we discuss number of large-scale proteomics and transcriptomics studies that have been performed using CD34 cells.
跨膜磷酸糖蛋白蛋白 CD34 传统上被认为是造血祖细胞的标志物。其在这些细胞上的表达已被用于各种血液系统疾病的细胞治疗应用。最近，CD34 的表达也已在非造血来源的细胞上被报道。这些细胞包括体细胞，如内皮细胞、成纤维细胞和间质细胞，以及再生干细胞，如角膜基质细胞、肌肉卫星细胞和肌肉来源的干细胞。此外，一些癌症干细胞 (CSC) 上也报道了其表达。迄今为止，该分子的功能作用已被涉及到多种细胞过程，包括细胞黏附、信号转导和祖细胞表型的维持。然而，对于该分子的完整理解，包括其发育起源、胚胎联系和相关功能，还远未完成。在这里，我们根据文献综述，回顾了我们对 CD34 分子结构和假定功能的现有理解。我们还探查了各种生物数据库，以检索与 CD34 表达和相关分子功能相关的数据。这些信息综合起来，很可能为这些细胞的细胞治疗提供适宜性。此外，我们还涵盖了来自不同谱系的 CD34 细胞的现有细胞治疗和推测的细胞治疗应用。我们还试图在这里推测 CD34 在 CSC 上的作用。最后，我们讨论了使用 CD34 细胞进行的一些大规模蛋白质组学和转录组学研究。
