大肠杆菌核糖体的30 S亚基。其组成蛋白的拓扑模型。

The 30 S subunit of the Escherichia coli ribosome. Topographical model of its component proteins.

作者信息

Cornick G G, Kretsinger R H

出版信息

Biochim Biophys Acta. 1977 Feb 3;474(3):398-410. doi: 10.1016/0005-2787(77)90269-6.

Abstract

This topographical model of the proteins of the 30 S subunit of the Escherichia coli ribosome was built to be consistent with the 37 published spectroscopic and chemical experiments that indicate proximity and with the two neutron diffraction experiments that indicate S3 and S7 as well as S2 and S5 to be separated by 110 A. The model is quite consistent with the protein arrangement suggested by assembly pathways, the various RNA binding sites, and the streptomycin-associated proteins, This consistency is encouraging since none of these data were considered during the construction of the model. The model differs significantly from those proposed by Traut et at. ((1974) Ribosomes 271-308) and by Tischendorf et al. ((1975) Proc. Natl. Acad. Sei. U.S. 72, 4820-4824).

摘要

构建大肠杆菌核糖体30 S亚基蛋白质的这种拓扑模型，是为了与37个已发表的表明接近程度的光谱和化学实验以及两个表明S3和S7以及S2和S5相隔110埃的中子衍射实验相一致。该模型与组装途径、各种RNA结合位点以及与链霉素相关的蛋白质所暗示的蛋白质排列相当一致。这种一致性令人鼓舞，因为在构建模型过程中并未考虑这些数据中的任何一个。该模型与Traut等人（(1974) Ribosomes 271 - 308）以及Tischendorf等人（(1975) Proc. Natl. Acad. Sei. U.S. 72, 4820 - 4824）提出的模型有显著差异。