Engelman D M, Moore P B, Schoenborn B P
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3888-92. doi: 10.1073/pnas.72.10.3888.
Neutron scattering measurements done on E. coli 30S ribosomal subunit specimens in which specific pairs of proteins were deuterated have enabled us to estimate the distances between the labeled proteins. The distances between centers of gravity of three protein pairs have been determined: S2-S5 (105 A), S3-S7 (115 A), and S5-S8 (35 A). A method for extracting shape information about these proteins from the neutron scattering profiles is demonstrated. The method shows that S5 and S8 are compact and S2 is extended.
对大肠杆菌30S核糖体亚基标本进行的中子散射测量,其中特定的蛋白质对被氘化,这使我们能够估计标记蛋白质之间的距离。已经确定了三对蛋白质重心之间的距离:S2 - S5(105埃)、S3 - S7(115埃)和S5 - S8(35埃)。展示了一种从中子散射谱中提取这些蛋白质形状信息的方法。该方法表明S5和S8是紧密的,而S2是伸展的。