Department of Neurology, Faculty of Medicine, Tokat Gaziosmanpasa University, Tokat, Turkey.
Department of Physiology, Faculty of Medicine, Tokat Gaziosmanpasa University, Tokat, Turkey.
Epilepsy Res. 2020 Jan;159:106262. doi: 10.1016/j.eplepsyres.2019.106262. Epub 2019 Dec 23.
Epilepsy is a disease characterized by seizures which impair human life considerably. Vitamin D is of different systemic effects on metabolism and its deficiency is known to have a high prevalence among epilepsy patients. Paricalcitol, a vitamin D receptor agonist, has relatively fewer side effects. This study aimed to investigate the anticonvulsant effect of vitamin D (cholecalciferol) and paricalcitol on penicillin-induced epileptiform activity.
21 male Wistar rat weighing 180-240 g were used. After anesthetized by 1.25 g/kg urethane intraperitoneally (i.p.), rats were placed in the stereotaxic frame and tripolar electrodes were placed on the skull. The single microinjection of penicillin (2.5 μl, 500 IU, i.c.) into left sensorimotor cortex induced epileptiform activity. A single dose of 60.000 IU/kg (i.p.) vitamin D was administered 14 days before intracortical penicillin (500 IU) injection. Paricalcitol (10 μg/kg, i.p.) was administered 30 min before intracortical penicillin (500 IU) administration and recorded for the following 180 min.
Vitamin D pretreatment and paricalcitol diminished the frequency of epileptiform activity (p < 0.001) without changing the amplitude (p > 0.05) compared to the penicillin-injected group. Vitamin D pretreatment and paricalcitol led to an important delay in the onset of penicillin-induced epileptiform activity (p < 0.001 and p < 0.05, respectively). Vitamin D increased the latency of penicillin-induced epileptic activity compared to paricalcitol group (p < 0.001).
Results indicate that vitamin D and paricalcitol decreased the frequency and increased the latency of the penicillin-induced epileptic activity. Vitamin D was more effective than paricalcitol.
癫痫是一种以发作为特征的疾病，严重影响人类生活。维生素 D 对代谢有不同的全身作用，其缺乏在癫痫患者中很常见。维生素 D 受体激动剂帕立骨化醇的副作用相对较少。本研究旨在探讨维生素 D（胆钙化醇）和帕立骨化醇对青霉素诱导的癫痫样活动的抗惊厥作用。
使用 21 只雄性 Wistar 大鼠，体重 180-240g。大鼠腹腔内注射 1.25g/kg 乌拉坦麻醉后，置于立体定向框架内，颅骨上放置三极电极。将青霉素（2.5μl，500IU，脑室内）单次微量注射到左侧感觉运动皮层诱导癫痫样活动。在脑室内注射青霉素（500IU）前 14 天，给予大鼠单次剂量 60000IU/kg（腹腔内）维生素 D。帕立骨化醇（10μg/kg，腹腔内）在脑室内注射青霉素（500IU）前 30 分钟给予，并记录随后的 180 分钟。
与青霉素注射组相比，维生素 D 预处理和帕立骨化醇降低了癫痫样活动的频率（p<0.001），而不改变振幅（p>0.05）。维生素 D 预处理和帕立骨化醇分别导致青霉素诱导的癫痫样活动发作时间明显延迟（p<0.001 和 p<0.05）。与帕立骨化醇组相比，维生素 D 增加了青霉素诱导的癫痫活动的潜伏期（p<0.001）。
结果表明，维生素 D 和帕立骨化醇降低了青霉素诱导的癫痫样活动的频率，并增加了潜伏期。维生素 D 比帕立骨化醇更有效。
