Department of Neurology, Faculty of Medicine, Tokat Gaziosmanpasa University, Tokat, Turkey.
Department of Physiology, Faculty of Medicine, Tokat Gaziosmanpasa University, Tokat, Turkey.
Neurosci Lett. 2021 May 14;753:135848. doi: 10.1016/j.neulet.2021.135848. Epub 2021 Apr 1.
The aim of the present study was to examine the effects of different quercetin pretreatment doses on focal epileptiform activity induced by penicillin in adult male rat cortex.
Twenty-eight male Wistar rats weighing 200-235 g were randomly divided into four groups: control (only penicillin-injected group) and penicillin + 25, 50 or 100 mg/kg quercetin doses. All quercetin-treated rats had a daily single dose of 25, 50 or 100 mg/kg intraperitoneally administered quercetin for 21 days, and the last dose was given 30 min before the penicillin injection. Epileptiform activity was induced by a single intracortical (i.c.) microinjection of penicillin (500 units/2.5 μl) into left motor cortex. After penicillin injection ECoG was recorded for the following 180 min.
Quercetin pretreatments of 25, 50 and 100 mg/kg significantly increased the duration of latency (initial spike activity) and decreased spike frequency of the epileptiform activity compared to the control group (p < 0.05). Duration of latency was significantly longer in 25 mg/kg quercetin pretreatment group compared to 100 mg/kg group (p < 0.05). Spike amplitude of epileptiform activity was not different in the study groups (p > 0.05).
Quercetin had an anticonvulsant activity in penicillin-induced focal seizure model in the present study. In addition, lower quercetin doses had highest anticonvulsant effect in this model.
本研究旨在探讨不同槲皮素预处理剂量对青霉素诱导成年雄性大鼠皮质局灶性癫痫样活动的影响。
28 只雄性 Wistar 大鼠，体重 200-235g，随机分为四组：对照组（仅注射青霉素组）和青霉素+25、50 或 100mg/kg 槲皮素剂量组。所有槲皮素处理组大鼠均每日腹腔注射 25、50 或 100mg/kg 槲皮素一次，连续 21 天，最后一次剂量在注射青霉素前 30 分钟给予。通过向左侧运动皮质单次皮质内（i.c.）微量注射青霉素（500 单位/2.5μl）诱导癫痫样活动。青霉素注射后，记录 ECoG 180 分钟。
与对照组相比，25、50 和 100mg/kg 槲皮素预处理显著延长潜伏期（初始棘波活动）持续时间，并降低癫痫样活动的棘波频率（p<0.05）。与 100mg/kg 槲皮素预处理组相比，25mg/kg 槲皮素预处理组潜伏期显著延长（p<0.05）。各组癫痫样活动的棘波幅度无差异（p>0.05）。
在本研究中，槲皮素对青霉素诱导的局灶性癫痫发作模型具有抗惊厥作用。此外，在该模型中，较低剂量的槲皮素具有最高的抗惊厥作用。
