Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science &Technology), Ministry of Education, Tianjin Key Lab of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
School of Life Science, Shandong University of Technology, Zibo 255049, PR China.
Carbohydr Polym. 2020 Feb 15;230:115605. doi: 10.1016/j.carbpol.2019.115605. Epub 2019 Nov 11.
Chitin is the second most abundant renewable polymer on earth, and its deacetylated derivative, chitosan, is a highly useful biopolymer. This work studied for the first time the application of ionic liquid (IL) tetrabutylammonium hydroxide ([TBA][OH]) and chitin deacetylase from Rhodococcus equi CGMCC14861 (ReCDA) for the efficient conversion of chitin into chitosan at room temperature. Results confirmed that chitin had good solubility in 18 wt% aqueous [TBA][OH] solution at 80 ℃. In addition, efficient chitin deactylation was observed with high concentrations of [TBA][OH] exceeding 12 wt% and showing potential application in chitin conversion. ReCDA activity on chitin was activated by [TBA][OH] pretreatment. Sequential and simultaneous strategies were also compared, and the results showed that the simultaneous one-pot deacetylation provided the highest acetic acid yield of 3.78 mg/g chitin powder after 24 h. This study serves as a guide for the dissolution and deacetylation of chitin to produce high value-added chitosan products.
壳聚糖是地球上第二大丰富的可再生聚合物，其脱乙酰衍生物壳聚糖是一种非常有用的生物聚合物。本研究首次探索了离子液体（IL）四丁基氢氧化铵([TBA][OH])和来自马红球菌 CGMCC14861 的壳聚糖脱乙酰酶（ReCDA）在室温下高效转化壳聚糖为壳聚糖的应用。结果证实，壳聚糖在 80℃下于 18wt%的[TBA][OH]水溶液中有良好的溶解性。此外，当[TBA][OH]浓度超过 12wt%时，观察到高效的壳聚糖脱乙酰化，这表明其在壳聚糖转化方面具有潜在的应用价值。[TBA][OH]预处理激活了 ReCDA 对壳聚糖的活性。还比较了顺序和同时两种策略，结果表明，在 24 小时后，同时一锅法脱乙酰化提供了最高的 3.78mg/g 壳聚糖粉末的乙酸产量。本研究为溶解和脱乙酰化壳聚糖以生产高附加值壳聚糖产品提供了指导。
