Germoush Mousa O, Elgebaly Hassan A, Hassan Sherif, Kamel Emadeldin M, Bin-Jumah May, Mahmoud Ayman M
Biology Department, College of Science, Jouf University, Sakaka 2014, Saudi Arabia.
Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef 62514, Egypt.
Antioxidants (Basel). 2019 Dec 26;9(1):22. doi: 10.3390/antiox9010022.
Seaweeds are rich in structurally diverse bioactive compounds with promising therapeutic effects. This study aimed to isolate and identify terpenes from the brown alga and to investigate its antidiabetic activity, pointing to the possible involvement of peroxisome proliferator-activated receptor (PPAR)γ. Type 2 diabetes was induced by feeding rats a high fat diet (HFD) for 4 weeks followed by injection of 35 mg/kg streptozotocin (STZ). The diabetic rats received extract (PPE; 50, 100 and 200 mg/kg) for 4 weeks and samples were collected for analyses. HFD/STZ-induced rats showed hyperglycemia, dyslipidemia, impaired glucose tolerance, decreased insulin, and increased HbA1c and HOMA-IR. PPE ameliorated hyperglycemia and dyslipidemia, and improved glucose tolerance and insulin sensitivity in diabetic rats. Treatment with PPE increased hepatic hexokinase activity and glycogen, suppressed glucose-6-phosphatase, fructose-1,6-biphosphatase, and glycogen phosphorylase, and attenuated oxidative stress, inflammation, and liver injury and lipid infiltration in HFD/STZ-induced rats. In addition, PPE boosted antioxidants and upregulated PPARγ gene and protein expression in the liver of diabetic rats. Phytochemical investigation resulted in the isolation of six terpenes from PPE and in silico analysis revealed their binding affinity toward PPARγ. In conclusion, -derived terpenes attenuated hyperglycemia, dyslipidemia, oxidative stress, and inflammation, and improved insulin sensitivity and carbohydrate metabolism in type 2 diabetic rats. These beneficial effects are mediated via PPARγ activation. However, further studies to explore the exact mechanisms underlying the antidiabetic effect of PPE are recommended.
海藻富含结构多样且具有潜在治疗作用的生物活性化合物。本研究旨在从褐藻中分离和鉴定萜类化合物，并研究其抗糖尿病活性，探讨过氧化物酶体增殖物激活受体（PPAR）γ可能的参与情况。通过给大鼠喂食高脂饮食（HFD）4周，随后注射35mg/kg链脲佐菌素（STZ）诱导2型糖尿病。糖尿病大鼠接受提取物（PPE；50、100和200mg/kg）治疗4周，并收集样本进行分析。HFD/STZ诱导的大鼠出现高血糖、血脂异常、糖耐量受损、胰岛素降低，以及糖化血红蛋白（HbA1c）和胰岛素抵抗指数（HOMA-IR）升高。PPE改善了糖尿病大鼠的高血糖和血脂异常，提高了糖耐量和胰岛素敏感性。PPE治疗增加了肝脏己糖激酶活性和糖原含量，抑制了葡萄糖-6-磷酸酶、果糖-1,6-二磷酸酶和糖原磷酸化酶，并减轻了HFD/STZ诱导大鼠的氧化应激、炎症、肝损伤和脂质浸润。此外，PPE增强了抗氧化剂，并上调了糖尿病大鼠肝脏中PPARγ基因和蛋白表达。植物化学研究从PPE中分离出六种萜类化合物，计算机模拟分析揭示了它们对PPARγ的结合亲和力。总之，源自褐藻的萜类化合物减轻了2型糖尿病大鼠的高血糖、血脂异常、氧化应激和炎症，改善了胰岛素敏感性和碳水化合物代谢。这些有益作用是通过激活PPARγ介导的。然而，建议进一步研究以探索PPE抗糖尿病作用的确切机制。
