das Neves Carlos G, Johansson Wensman Jonas, Nymo Ingebjørg Helena, Skjerve Eystein, Alenius Stefan, Tryland Morten
Norwegian Veterinary Institute, N-0106 Oslo/N-9010 Tromsø, Norway.
Faculty of Health Sciences, UiT The Arctic University of Norway, N-9037 Tromsø, Norway.
Viruses. 2019 Dec 26;12(1):29. doi: 10.3390/v12010029.
Members of the Pestivirus genus (family ) cause severe and economically important diseases in livestock. Serological studies have revealed the presence of pestiviruses in different cervid species, including wild and semi-domesticated Eurasian tundra reindeer. In this retrospective study, serum samples collected between 2006 and 2008 from 3339 semi-domesticated Eurasian reindeer from Finnmark County, Norway, were tested for anti-pestivirus antibodies using an enzyme linked immunosorbent assay (ELISA) and a subset of these by virus neutralization test (VNT). A seroprevalence of 12.5% was found, varying from 0% to 45% among different herding districts, and 20% in western Finnmark, as compared to 1.7% in eastern Finnmark. Seroprevalence increased with age. Pestivirus-specific RNA was not detected in any of the 225 serum samples tested by real-time RT-PCR. Based on VNT results, using a panel of one bovine viral diarrhea virus (BVDV) strain and two border disease virus (BDV) strains, the virus is most likely a reindeer-specific pestivirus closely related to BDV. A characterization of the causative virus and its pathogenic impact on reindeer populations, as well as its potential to infect other domestic and wild ruminants, should be further investigated.
瘟病毒属（科）的成员可在牲畜中引发严重且具有经济重要性的疾病。血清学研究已揭示在包括野生和半驯化的欧亚苔原驯鹿在内的不同鹿类物种中存在瘟病毒。在这项回顾性研究中，对2006年至2008年间从挪威芬马克郡采集的3339份半驯化欧亚驯鹿的血清样本，使用酶联免疫吸附测定（ELISA）检测抗瘟病毒抗体，并对其中一部分样本进行病毒中和试验（VNT）。结果发现血清阳性率为12.5%，在不同放牧区中从0%到45%不等，在芬马克郡西部为20%，而在芬马克郡东部为1.7%。血清阳性率随年龄增长而升高。通过实时逆转录聚合酶链反应（RT-PCR）检测的225份血清样本中均未检测到瘟病毒特异性RNA。基于VNT结果，使用一组一株牛病毒性腹泻病毒（BVDV）毒株和两株边境病病毒（BDV）毒株进行检测，该病毒很可能是一种与BDV密切相关的驯鹿特异性瘟病毒。对致病病毒及其对驯鹿种群的致病影响，以及其感染其他家养和野生反刍动物的可能性，应进一步展开研究。
