Center for Interdisciplinary Research in Health, Institute of Health Sciences, Universidade Católica Portuguesa, Porto, Portugal.
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, 4710-057, Portugal.
Sci Rep. 2019 Dec 30;9(1):20289. doi: 10.1038/s41598-019-56827-8.
Stress responses are associated with elevations in corticosterone levels and, as a consequence, increases in glutamate in the central nervous system which can lead to neurological impairment. Ceftriaxone promotes glutamate transport and has been used to reduce glutamate toxicity, but so far it is not known whether ceftriaxone is able to reverse the effects of corticosterone administration. Here we describe the separate and combined effects of acute ceftriaxone and acute corticosterone administration in local field potentials (LFPs) recorded from the somatosensory cortex (S1) of anesthetized mice. For this, LFPs were recorded from groups of anesthetized mice injected with saline, corticosterone, ceftriaxone, or both. Comparison of global state maps, and their displacements, as measured by ratios of different frequency bands (Ratio 1: 0.5-20 Hz/0.5-45 Hz; and Ratio 2: 0.5-4.5 Hz/0.5-9 Hz) revealed distinct and opposite effects for corticosterone and for ceftriaxone. Corticosterone specifically increased the displacement in Ratio 2, while ceftriaxone decreased it; in addition, when both corticosterone and ceftriaxone were injected, Ratio 2 displacement values were again similar to those of the control group. The present results suggest that ceftriaxone and corticosterone modulate specific frequency bands in opposite directions and reveal a potential role for ceftriaxone in counteracting the effects of corticosterone.
应激反应与皮质酮水平的升高有关，而皮质酮水平的升高会导致中枢神经系统中谷氨酸的增加，从而导致神经损伤。头孢曲松可促进谷氨酸转运，并已被用于减少谷氨酸毒性，但目前尚不清楚头孢曲松是否能够逆转皮质酮给药的影响。在这里，我们描述了急性头孢曲松和急性皮质酮给药对麻醉小鼠体感皮层（S1）记录的局部场电位（LFPs）的单独和联合作用。为此，我们从注射生理盐水、皮质酮、头孢曲松或两者的麻醉小鼠组中记录 LFPs。通过比较全局状态图及其位移（通过不同频段的比率测量：Ratio 1：0.5-20 Hz/0.5-45 Hz；Ratio 2：0.5-4.5 Hz/0.5-9 Hz），发现皮质酮和头孢曲松具有明显相反的作用。皮质酮特异性地增加了 Ratio 2 的位移，而头孢曲松则降低了它；此外，当同时注射皮质酮和头孢曲松时，Ratio 2 的位移值又与对照组相似。本研究结果表明，头孢曲松和皮质酮以相反的方向调节特定的频段，并揭示了头孢曲松在抵消皮质酮作用方面的潜在作用。
