临床和重症监护病房环境分离的铜绿假单胞菌毒力因子检测及耐药模式分析。

Detection of Virulence Factors and Antibiotic Resistance Pattern of Clinical and Intensive Care Unit Environmental Isolates of Pseudomonas aeruginosa.

机构信息

Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran.

Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.

出版信息

Infect Disord Drug Targets. 2020;20(5):758-762. doi: 10.2174/1871526520666191231124717.

DOI:10.2174/1871526520666191231124717

PMID:31889501

Abstract

BACKGROUND

Pseudomonas aeruginosa is a gram-negative non-glucose fermenting aerobic bacteria and an opportunistic pathogen in humans and animals. The present study was carried out to investigate the distribution of virulence factors and antibiotic resistance properties of P. aeruginosa isolated from patients and intensive care unit (ICU) environment.

MATERIAL AND METHODS

A total of 116 P. aeruginosa isolated from patients and ICU environment were collected from Besat hospital in Hamadan, the West of Iran. P. aeruginosa isolates were analyzed based on the presence of the virulence factors encoding genes included exoA, exoS, exoU, and algD using polymerase chain reaction (PCR). Antimicrobial susceptibility test was performed using a disk diffusion method.

RESULTS

The results showed the prevalence of exoA 33 (56.9%), exoS 21 (36.20%), exoU 37 (63.8%), and algD 35 (60.34%) genes in ICU environment P. aeruginosa strains and exo A 23 (39.25%), exoS 25 (43.1%), exoU 40(68.98%), and algD 25 (43.1%) genes in clinical isolates of P. aeruginosa. High resistance levels of the clinical and ICU environment isolate to ampicillinsulbactam (100%), were also observed.

CONCLUSION

Our findings should raise awareness about antibiotic resistance in hospitalized patients in Iran. Clinicians should exercise caution in prescribing antibiotics, especially in cases of human infections.

摘要

背景

铜绿假单胞菌是一种革兰氏阴性非葡萄糖发酵需氧细菌，也是人类和动物的机会性病原体。本研究旨在调查从患者和重症监护病房（ICU）环境中分离的铜绿假单胞菌的毒力因子分布和抗生素耐药特性。

材料和方法

从伊朗西部哈马丹 Besat 医院收集了 116 株来自患者和 ICU 环境的铜绿假单胞菌分离株。使用聚合酶链反应（PCR）分析了携带编码外毒素 A（exoA）、外毒素 S（exoS）、外毒素 U（exoU）和 algD 等毒力因子的基因。采用纸片扩散法进行抗生素药敏试验。

结果

结果显示，ICU 环境中铜绿假单胞菌分离株的 exoA 基因流行率为 33（56.9%），exoS 基因流行率为 21（36.20%），exoU 基因流行率为 37（63.8%），algD 基因流行率为 35（60.34%）；临床分离株的 exoA 基因流行率为 23（39.25%），exoS 基因流行率为 25（43.1%），exoU 基因流行率为 40（68.98%），algD 基因流行率为 25（43.1%）。临床和 ICU 环境分离株对氨苄西林/舒巴坦的耐药率均高达 100%。