Badamchi Ali, Masoumi Hossein, Javadinia Shima, Asgarian Ramin, Tabatabaee Azardokht
Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
Research Center of Pediatric Infectious Diseases, Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.
Microb Pathog. 2017 Jun;107:44-47. doi: 10.1016/j.micpath.2017.03.009. Epub 2017 Mar 16.
Although a vast majority of Urinary tract infections (UTIs) are caused by E. coli, epidemiological reports have indicated an increasing rate of such infections caused by some other opportunistic organisms including Pseudomonas aeruginosa. Antimicrobial susceptibility and pathogenesis mechanisms of P. aeruginosa are poorly understood. The aim of this study was to detect some virulence factor genes and antimicrobial susceptibility patterns of P. aeruginosa isolates detected in patients with UTI, in children hospital of Tehran, Tehran, Iran. Eighty-four Pseudomonas aeruginosa were isolated. Then, the presence of six virulence genes, in the genome of the isolates was evaluated using PCR amplifications techniques. Finally, antimicrobial susceptibility pattern of the isolates was determined by disk diffusion method. According to the results, lasB was the most prevalent virulence gene that could be detected in the P. aeruginosa isolates (92.9%) used in this study. This was followed by aprA (81.2%), toxA (69.4%), and algD (60%) genes. Two genes, plcH and plcN, were detected in about 38.8% of the isolates. Additionally, Imipenem was found as the most active agent against the P. aeruginosa isolates used in this research. However, Cefotaxime resistance was observed in most of the isolates. Our P. aeruginosa isolates exhibited a great degree of heterogeneity not only in their virulence genes but also in their antimicrobial susceptibility profiles. Imipenem therapies tend to be among the best choices in the management of UTI caused by P. aeruginosa. As a conclusion, assessment of antimicrobial susceptibility pattern and also analyzing the virulence factors can be highly helpful to develop effective treatment strategies against P. aeruginosa urinary infections.
尽管绝大多数尿路感染（UTIs）是由大肠杆菌引起的，但流行病学报告显示，由包括铜绿假单胞菌在内的其他一些机会性微生物引起的此类感染率正在上升。人们对铜绿假单胞菌的抗菌药敏性和致病机制了解甚少。本研究的目的是检测在伊朗德黑兰德黑兰儿童医院尿路感染患者中分离出的铜绿假单胞菌菌株的一些毒力因子基因和抗菌药敏模式。共分离出84株铜绿假单胞菌。然后，使用PCR扩增技术评估分离株基因组中六个毒力基因的存在情况。最后，通过纸片扩散法确定分离株的抗菌药敏模式。根据结果，lasB是本研究中在铜绿假单胞菌分离株中最普遍可检测到的毒力基因（92.9%）。其次是aprA（81.2%）、toxA（69.4%）和algD（60%）基因。约38.8%的分离株中检测到plcH和plcN两个基因。此外，亚胺培南被发现是对本研究中使用的铜绿假单胞菌分离株最有效的药物。然而，大多数分离株对头孢噻肟耐药。我们的铜绿假单胞菌分离株不仅在毒力基因上，而且在抗菌药敏谱上都表现出很大程度的异质性。亚胺培南治疗往往是治疗由铜绿假单胞菌引起的尿路感染的最佳选择之一。总之，评估抗菌药敏模式以及分析毒力因子对于制定针对铜绿假单胞菌泌尿系统感染的有效治疗策略可能非常有帮助。
