KTMT5的抗癌活性及代谢物谱数据
Anticancer activity and metabolite profiling data of KTMT5.
作者信息
Tapfuma Kudzanai Ian, Sebola Tendani Edith, Uche-Okereafor Nkemdinma, Koopman Jody, Hussan Raeesa, Makatini Maya Mellisa, Mekuto Lukhanyo, Mavumengwana Vuyo
机构信息
DST/NRF Centre of Excellence for Biomedical Tuberculosis Research and SAMRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Stellenbosch University, PO Box 19063, Tygerberg, 7505, Cape Town, South Africa.
Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, PO Box 17011, Doornfontein, Johannesburg, 2028, South Africa.
出版信息
Data Brief. 2019 Dec 7;28:104959. doi: 10.1016/j.dib.2019.104959. eCollection 2020 Feb.
Fungi are ubiquitous, they proliferate even in environments with toxic pollutants that are otherwise harmful to other eukaryotes. This article presents data of fungi which were isolated from gold mine tailings and identified by DNA sequencing of their inter transcribed spacer regions 1 and 2. Five fungal isolates were identified, among which the crude extract of KTMT5 was investigated for anticancer activity on A549 (lung carcinoma) and UMG87 (glioblastoma) cell lines. Untargeted metabolite profiling of the crude extract of KTMT5 was performed using liquid chromatography quadrupole time of flight tandem mass spectrometry (LC-QTOF-MS/MS) and a molecular network generated using the online workflow on the Global Natural Product Social molecular networking (GNPS) website. DNA sequencing showed that all fungal isolates belonged to phylum Ascomycota with the genus Penicillium representing 75% of the fungal isolates. KTMT5 which was selected for further experiments showed significant anticancer activity against UMG87 cells with a calculated IC value of 44.23 μg/mL in the MTS assay, while the real time xCELLigence assay showed dose-dependent anticancer activity at 50 and 100 μg/mL. Metabolite profiling revealed the presence of several known metabolites in the crude extract of KTMT5 and molecular networking showed the relationships among these metabolites.
真菌无处不在，即使在含有对其他真核生物有害的有毒污染物的环境中也能繁殖。本文展示了从金矿尾矿中分离出的真菌的数据，并通过对其转录间隔区1和2进行DNA测序来鉴定这些真菌。共鉴定出5株真菌分离株，其中对KTMT5的粗提物进行了对A549（肺癌）和UMG87（胶质母细胞瘤）细胞系的抗癌活性研究。使用液相色谱四极杆飞行时间串联质谱（LC-QTOF-MS/MS）对KTMT5的粗提物进行非靶向代谢物谱分析，并利用全球天然产物社会分子网络（GNPS）网站上的在线工作流程生成分子网络。DNA测序表明，所有真菌分离株均属于子囊菌门，其中青霉属占真菌分离株的75%。被选用于进一步实验的KTMT5在MTS试验中对UMG87细胞显示出显著的抗癌活性，计算得出的IC值为44.23μg/mL，而实时xCELLigence试验显示在50和100μg/mL时具有剂量依赖性抗癌活性。代谢物谱分析揭示了KTMT5粗提物中存在几种已知代谢物，分子网络显示了这些代谢物之间的关系。
Zotero 插件