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肽和蛋白质的选择——mRNA 展示的优势。

Selection of Peptides and Proteins-Advantages of mRNA Display.

机构信息

Department of Biochemistry, Molecular Biology and Biophysics & BioTechnology Institute , University of Minnesota , 1479 Gortner Avenue , St. Paul , Minnesota 55108 , United States.

Department of Molecular, Cellular, and Developmental Biology & Cooperative Institute for Research in Environmental Sciences , University of Colorado Boulder , Boulder , Colorado 80309 , United States.

出版信息

ACS Synth Biol. 2020 Feb 21;9(2):181-190. doi: 10.1021/acssynbio.9b00419. Epub 2020 Jan 14.

DOI:10.1021/acssynbio.9b00419
PMID:31891492
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8203280/
Abstract

mRNA display is a robust selection technique that allows the selection of peptides and proteins with desired functions from libraries of trillions of variants. mRNA display relies upon a covalent linkage between a protein and its encoding mRNA molecule; the power of the technique stems from the stability of this link, and the large degree of control over experimental conditions afforded to the researcher. This article describes the major advantages that make mRNA display the method of choice among comparable and methods, including cell-surface display, phage display, and ribosomal display. We also describe innovative techniques that harness mRNA display for directed evolution, protein engineering, and drug discovery.

摘要

mRNA 展示是一种强大的筛选技术，它允许从数万亿个变体库中选择具有所需功能的肽和蛋白质。mRNA 展示依赖于蛋白质与其编码的 mRNA 分子之间的共价连接；该技术的强大之处在于这种连接的稳定性，以及研究人员对实验条件的高度控制。本文介绍了使 mRNA 展示成为可比方法中首选方法的主要优势，包括细胞表面展示、噬菌体展示和核糖体展示。我们还描述了利用 mRNA 展示进行定向进化、蛋白质工程和药物发现的创新技术。

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