Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, 453007, PR China.
Henan Key Laboratory for Environmental Pollution Control, Key Laboratory for Yellow River and Huai River Water Environmental Pollution and Control, Ministry of Education, School of Environment, Henan Normal University, Xinxiang, 453007, PR China.
Talanta. 2020 Mar 1;209:120519. doi: 10.1016/j.talanta.2019.120519. Epub 2019 Oct 31.
In this study, facile fabrication of three-dimensional (3D) pompon-like gold/zinc oxide (Au/ZnO) porous microspheres by hydrothermal procedure was investigated. These microspheres were utilized as solid phase microextraction (SPME) coating for determination of volatile fatty acids (VFAs) from foot odor coupling with gas chromatography-mass spectrometry (GC-MS). SEM and TEM characterizations showed that as-prepared material was composed of 1D porous nanowires and presented a uniform coating on stainless-steel wire. The extraction of VFAs including propanoic acid, butyric acid, isobutanoic acid, isovaleric acid, hexanoic acid, and heptylic acid was carried out by headspace model after sampling from human foot using cotton wool strips. Following optimization of extraction parameters including extraction temperature and time and desorption temperature and time, the as-prepared SPME coating presented better extraction efficiency than commercial DVB/CAR/PDMS fiber towards all the VFAs due to its excellent properties. Under the optimized conditions, the method exhibited good linearity (0.5-200 ng) with regression coefficients (R) ranging from 0.9836 to 0.9981 for all the analytes. The limits of detection ranged from 0.017 to 0.098 ng. Single fiber repeatability varied from 6.5% to 11.2% and the fiber-to-fiber reproducibility ranged from 8.6% to 12.3%. The proposed method was successfully applied for extraction and determination of VFAs from foot odor after sampling using cotton wool strips.
在这项研究中，通过水热法成功制备了三维（3D）绒球状金/氧化锌（Au/ZnO）多孔微球。这些微球被用作固相微萃取（SPME）涂层，结合气相色谱-质谱联用（GC-MS）用于检测脚臭中的挥发性脂肪酸（VFAs）。SEM 和 TEM 表征表明，所制备的材料由 1D 多孔纳米线组成，在不锈钢丝上呈现均匀的涂层。通过使用棉绒条从人脚取样后，采用顶空模式提取丙酸、丁酸、异丁酸、异戊酸、己酸和庚酸等 VFAs。在优化萃取参数（包括萃取温度和时间以及解吸温度和时间）后，与商业的 DVB/CAR/PDMS 纤维相比，所制备的 SPME 涂层对所有 VFAs 都具有更好的萃取效率，因为它具有优异的性能。在优化条件下，该方法对所有分析物均表现出良好的线性（0.5-200ng），相关系数（R）范围为 0.9836 至 0.9981。检出限范围为 0.017 至 0.098ng。单根纤维的重复性变化为 6.5%至 11.2%，纤维间的重现性范围为 8.6%至 12.3%。该方法成功应用于棉绒条取样后脚臭中 VFAs 的提取和测定。
