Laboratório de Quimiometria em Ciências Naturais, Departamento de Química, Universidade Estadual de Londrina, CP 6001, 86051-990, Londrina-PR, Brazil.
Laboratório de Quimiometria em Ciências Naturais, Departamento de Química, Universidade Estadual de Londrina, CP 6001, 86051-990, Londrina-PR, Brazil; Instituto de Química, Universidade Estadual de Campinas, CP 6154, 13083-970, Campinas-SP, Brazil.
Talanta. 2020 Mar 1;209:120591. doi: 10.1016/j.talanta.2019.120591. Epub 2019 Nov 25.
Coffee is one of the most important commodities, showing sensitivity to environmental variations. The main effects and their interaction for two levels of atmospheric CO concentrations and two water regimes of a factorial design were investigated for the metabolic profiles of Coffea arabica raw beans using UV fingerprint analysis from a mixture design. UV fingerprint results obtained from pure ethanol and binary ethanol-dichloromethane mixtures showed the largest metabolic discriminations between CO levels and their extracts were investigated in detail. The biosynthesis of major metabolites, chlorogenic acids, cafestol, kahweol and caffeine were altered owing to environmental conditions. Higher amounts of chlorogenic acids and kahweol were observed in beans from unirrigated plants grown with enriched CO and irrigated ones at the current CO level. Water availability and CO concentration interaction affects the metabolite amounts. Besides a significant CO atmospheric effect water availability was a limiting factor for metabolite content only at current CO level, suggesting the successful metabolic coping of CO enriched Arabic coffee beans suffering future droughts.
咖啡是最重要的商品之一，对环境变化非常敏感。采用混合设计的紫外指纹分析，对两个大气 CO2浓度水平和两个水分条件的因子设计进行了主要效应及其互作研究，以考察阿拉比卡咖啡豆的代谢特征。从纯乙醇和二元乙醇-二氯甲烷混合物中获得的紫外指纹结果表明，CO2 水平之间存在最大的代谢差异，并且对其提取物进行了详细研究。由于环境条件的影响，主要代谢物绿原酸、咖啡醇、卡瓦酚和咖啡因的生物合成发生了改变。在未灌溉的植物中，富 CO2 条件下和目前 CO2 水平下灌溉的植物中，咖啡豆中的绿原酸和卡瓦酚含量更高。水分供应和 CO2 浓度的相互作用影响代谢物的含量。除了大气 CO2 的显著影响外，只有在目前 CO2 水平下，水分供应才是代谢物含量的限制因素，这表明富含 CO2 的阿拉伯咖啡豆在未来遭受干旱时，能够成功地进行代谢适应。
