In vitro evaluation of the hemostatic effect of method involving the combined use of Hydrofit and Spongel.

OBJECTIVE

We developed an effective hemostatic method using Hydrofit and a hemostatic gelatin sponge (Spongel). We evaluated the hemostatic effect in comparison to the conventional silicone sheet method.

METHODS

A simulated circuit was created using the pump of a Nipro ventricular assist system and a prosthetic graft. A hole was made in the graft by a needle and three hemostatic methods were applied: the silicone sheet method (SS) using Hydrofit and a silicone sheet, the bread and butter method (BB) using Hydrofit and a gelatin sponge instead of a silicone sheet, and French toast method (FT) using Hydrofit and a gelatin sponge over which water was poured before compression. The amount of leakage before and after the application each of the methods was measured according to the compression time.

RESULTS

In the 60 s compression, the amount of leakage after SS, BB, and FT was 0.4 ± 0.8, 0.2 ± 0.6, and 0 ± 0.0 ml, respectively, and FT showed no leakage. In the 30 s compression, the amount of leakage after SS, BB, and FT was 14.2 ± 27.9, 1.0 ± 3.2, and 7.8 ± 22.6 ml, respectively, and did not differ to a statistically significant extent.

CONCLUSIONS

The method of combining Hydrofit and Spongel could obtain reliable hemostasis in 60 s.