State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China.
Nanoscale. 2020 Jan 23;12(3):1673-1679. doi: 10.1039/c9nr07550j.
Ribonuclease H (RNase H), an intracellular ribonuclease, plays a crucial role in cellular processes and especially relates to many disease processes. Here, we report a novel signal amplification strategy based on an RNase H-powered DNA walking machine for specific and sensitive RNase H activity detection. The DNA walking machine is composed of a small quantity of DNA walker strands and abundant FAM-labeled DNA-RNA chimeric strands on a single gold nanoparticle (AuNP). RNase H can specifically degrade the RNA fragment in a DNA-RNA hybrid duplex and trigger the autonomous movement of a DNA walker strand on the AuNP surface. During this process, each step of the walking can release the FAM-labeled RNA from the surface of the AuNP, realizing the signal amplification for RNase H sensing. This method has been successfully utilized for RNase H activity detection in a complex system and applied for screening of related inhibitors. Therefore, our RNase H-powered DNA walking machine gives a novel platform for RNase H activity detection and RNase H-associated drug discovery.
核糖核酸酶 H（RNase H）是一种细胞内的核糖核酸酶，在细胞过程中发挥着关键作用，特别是与许多疾病过程有关。在这里，我们报告了一种基于 RNase H 驱动的 DNA 行走机器的新型信号放大策略，用于特定和敏感的 RNase H 活性检测。DNA 行走机器由少量的 DNA 行走链和大量的 FAM 标记的 DNA-RNA 嵌合体链组成，位于单个金纳米颗粒（AuNP）上。RNase H 可以特异性地降解 DNA-RNA 杂交双链中的 RNA 片段，并触发 AuNP 表面上的 DNA 行走链的自主运动。在这个过程中，行走的每一步都可以从 AuNP 表面释放 FAM 标记的 RNA，实现了用于 RNase H 传感的信号放大。该方法已成功用于复杂体系中 RNase H 活性的检测，并应用于相关抑制剂的筛选。因此，我们的 RNase H 驱动的 DNA 行走机器为 RNase H 活性检测和与 RNase H 相关的药物发现提供了一个新的平台。
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