Deparment of Bioprocess Engineering, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do, 54896, Republic of Korea.
Graduate School of Semiconductor and Chemical Engineering, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do, 54896, Republic of Korea.
Mol Biotechnol. 2020 Mar;62(3):185-191. doi: 10.1007/s12033-019-00235-w.
All eukaryotes have lysosomes which contain hydrolytic enzymes such as protease to degrade waste materials and cellular fragments. As a cellular organelle, lysosomes function as the digestive system of the cell, serving both to degrade material taken up from outside the cell and to digest obsolete components of the cell itself. Conversely, melanin has photochemical functions to protect tissue from the harmful effects of ultraviolet rays. However, too much of melanin leads to problems such as hyperpigmentation, requiring materials to maintain and control the amount of melanin. In this study, we found evidence of correlation between lysosome and melanin in a new eco-friendly material, MelanoDerm, a reconstituted 3D human skin model containing normal melanocytes and keratinocytes. Melanin content assay and cell viability were measured, using 2% kojic acid as positive control, while MelanoDerm was exposed to various concentrations of lysosome. Our results indicate that lysosome may be a useful cosmetic agent for the treatment of hyperpigmentation.
所有真核生物都有溶酶体，其中含有水解酶，如蛋白酶，以降解废物和细胞碎片。作为一种细胞细胞器，溶酶体起着细胞消化系统的作用，既能降解从细胞外摄取的物质，又能消化细胞自身老化的成分。相反，黑色素具有光化学功能，可以保护组织免受紫外线的有害影响。然而，过多的黑色素会导致色素沉着等问题，需要材料来维持和控制黑色素的含量。在这项研究中，我们在一种新的环保材料 MelanoDerm 中发现了溶酶体和黑色素之间的相关性，这是一种含有正常黑素细胞和成纤维细胞的重建 3D 人皮肤模型。使用 2%曲酸作为阳性对照，测量了黑色素含量测定和细胞活力，同时将 MelanoDerm 暴露于不同浓度的溶酶体中。我们的结果表明，溶酶体可能是一种有用的化妆品，用于治疗色素沉着过度。
