Catlin E A, Manganaro T F, Donahoe P K
Pediatric Surgical Research Laboratory, Massachusetts General Hospital, Boston 02114.
Am J Obstet Gynecol. 1988 Nov;159(5):1299-303. doi: 10.1016/0002-9378(88)90467-x.
Respiratory distress syndrome and associated pulmonary surfactant deficiency are more common in male neonates. Androgens have been shown to depress surfactant production. We tested the hypothesis that müllerian inhibiting substance, another fetal testicular product, might inhibit lung maturation measured as disaturated phosphatidylcholine accumulation. Initially, female fetal rat lungs were incubated with fetal testis or ovary or nanomolar concentrations of bovine müllerian inhibiting substance. Cultured lungs produced less disaturated phosphatidylcholine after incubation for 5 days with either testis (p = 0.012) or müllerian inhibiting substance than after coculture with ovary. In more comprehensive experiments, female lung fragments of 17.5 days' gestation, when incubated with nanomolar concentrations of bovine müllerian inhibiting substance or picomolar concentrations of human recombinant müllerian inhibiting substance, showed significant suppression of disaturated phosphatidylcholine accumulation (p less than 0.004) when compared with incubation with vehicle buffer. The suppression of surfactant accumulation produced in vitro by müllerian inhibiting substance, a potent male-specific fetal regressor, may be a factor in the increased susceptibility of male infants to respiratory distress syndrome.
呼吸窘迫综合征及相关的肺表面活性物质缺乏在男性新生儿中更为常见。雄激素已被证明会抑制表面活性物质的产生。我们检验了一种假说，即苗勒管抑制物质（另一种胎儿睾丸产物）可能会抑制以双饱和磷脂酰胆碱积累来衡量的肺成熟。最初，将雌性胎鼠肺与胎儿睾丸或卵巢或纳摩尔浓度的牛苗勒管抑制物质一起孵育。与卵巢共培养相比，用睾丸（p = 0.012）或苗勒管抑制物质孵育5天后，培养的肺产生的双饱和磷脂酰胆碱较少。在更全面的实验中，妊娠17.5天的雌性肺组织碎片，当与纳摩尔浓度的牛苗勒管抑制物质或皮摩尔浓度的人重组苗勒管抑制物质一起孵育时，与用赋形剂缓冲液孵育相比，双饱和磷脂酰胆碱的积累受到显著抑制（p < 0.004）。苗勒管抑制物质（一种强大的雄性特异性胎儿消退因子）在体外产生的对表面活性物质积累的抑制作用，可能是男婴对呼吸窘迫综合征易感性增加的一个因素。
