St. Petersburg State University, 198504 St. Petersburg, Russian Federation.
St. Petersburg State University, 198504 St. Petersburg, Russian Federation.
Adv Colloid Interface Sci. 2020 Feb;276:102086. doi: 10.1016/j.cis.2019.102086. Epub 2019 Dec 14.
The review discusses recent results on the adsorption of amyloid fibrils and protein microgels at liquid/fluid interfaces. The application of the shear and dilational surface rheology, atomic force microscopy and passive particle probe tracking allowed for elucidating characteristic features of the protein aggregate adsorption while some proposed hypothesis still must be examined by special methods for structural characterization. Although the distinctions of the shear surface properties of dispersions of protein aggregates from the properties of native protein solutions are higher than the corresponding distinctions of the dilational surface properties, the latter ones give a possibility to obtain new information on the formation of fibril aggregates at the water/air interface. Only the adsorption of BLG microgels and fibrils was studied in some details. The kinetic dependencies of the dynamic surface tension and dilational surface elasticity for aqueous dispersions of protein globules, protein microgels and purified fibrils are similar if the system does not contain flexible macromolecules or flexible protein fragments. In the opposite case the kinetic dependencies of the dynamic surface elasticity can be non-monotonic. The solution pH influences strongly the dynamic surface properties of the dispersions of protein aggregates indicating that the adsorption kinetics is controlled by an electrostatic adsorption barrier if the pH deviates from the isoelectric point. A special section of the review considers the possibility to apply kinetic models of nanoparticle adsorption to the adsorption of protein aggregates.
这篇综述讨论了最近在液体/流体界面上的淀粉样纤维和蛋白质微凝胶吸附的研究结果。应用剪切和拉伸表面流变学、原子力显微镜和被动粒子探针跟踪技术，能够阐明蛋白质聚集体吸附的特征，而一些提出的假设仍需通过特殊的结构表征方法进行检验。尽管蛋白质聚集体分散体的剪切表面性质与天然蛋白质溶液的性质之间的区别高于相应的拉伸表面性质之间的区别，但后者可以提供有关在水/气界面上形成纤维状聚集体的新信息。只有 BLG 微凝胶和纤维的吸附被详细研究过。如果体系中不含有柔性大分子或柔性蛋白质片段，则蛋白质球、蛋白质微凝胶和纯化纤维的水性分散体的动态表面张力和拉伸表面弹性的动力学依赖性是相似的。在相反的情况下，动态表面弹性的动力学依赖性可能是非单调的。溶液 pH 值强烈影响蛋白质聚集体分散体的动态表面性质，表明如果 pH 值偏离等电点，则吸附动力学受静电吸附势垒控制。综述的一个专门部分考虑了将纳米颗粒吸附的动力学模型应用于蛋白质聚集体吸附的可能性。
