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微小RNA-431通过靶向成纤维细胞生长因子9发挥乳腺癌肿瘤抑制作用。

MicroRNA-431 serves as a tumor inhibitor in breast cancer through targeting FGF9.

作者信息

Wang Wei, Dong Yan, Li Xiaoyan, Pan Yingying, Du Jiexin, Liu Daotong

机构信息

Department of General Surgery, Zhangqiu Maternity and Child Care Hospital, Jinan, Shandong 250200, P.R. China.

Breast Clinic Center, Qingdao Central Hospital, Qingdao, Shandong 266042, P.R. China.

出版信息

Oncol Lett. 2020 Jan;19(1):1001-1007. doi: 10.3892/ol.2019.11126. Epub 2019 Nov 20.

DOI:10.3892/ol.2019.11126
PMID:31897213
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6924186/
Abstract

Breast cancer has become an important public health problem. Moreover, the functions of microRNA-431 (miR-431) have been detected in human cancers other than breast cancer. Hence, we investigated the role of miR-431 in progression of breast cancer. RT-qPCR and Western blot analysis were performed to assess expression of miR-431 and genes. The regulatory mechanism of miR-431 was investigated using MTT, Transwell and luciferase reporter assay. Decreased miR-431 expression was identified in breast cancer, which was related to aggressive behavior. Furthermore, miR-431 restrained cell proliferation, metastasis and EMT in breast cancer. miR-431 induced apoptosis through enhancing Bax expression. In addition, miR-431 was found to directly target FGF9. Moreover, upregulation of FGF9 impaired the anti-tumor effect of miR-431 in breast cancer. miR-431 restrained cell viability and metastasis in breast cancer through targeting FGF9, indicating that miR-431 serves as a tumor inhibitor in breast cancer.

摘要

乳腺癌已成为一个重要的公共卫生问题。此外，微小RNA - 431（miR - 431）的功能已在乳腺癌以外的其他人类癌症中被检测到。因此，我们研究了miR - 431在乳腺癌进展中的作用。采用逆转录定量聚合酶链反应（RT - qPCR）和蛋白质免疫印迹分析来评估miR - 431和基因的表达。使用MTT法、Transwell实验和荧光素酶报告基因检测法研究miR - 431的调控机制。在乳腺癌中发现miR - 431表达降低，这与侵袭性行为有关。此外，miR - 431抑制乳腺癌细胞的增殖、转移和上皮 - 间质转化（EMT）。miR - 431通过增强Bax表达诱导细胞凋亡。此外，发现miR - 431直接靶向成纤维细胞生长因子9（FGF9）。而且，FGF9的上调削弱了miR - 431在乳腺癌中的抗肿瘤作用。miR - 431通过靶向FGF9抑制乳腺癌细胞的活力和转移，表明miR - 431在乳腺癌中起着肿瘤抑制因子的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14cc/6924186/929a0a89ea06/ol-19-01-1001-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14cc/6924186/1ef957590551/ol-19-01-1001-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14cc/6924186/4ef8545361ae/ol-19-01-1001-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14cc/6924186/468f2963c95b/ol-19-01-1001-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14cc/6924186/917aa3441e43/ol-19-01-1001-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14cc/6924186/929a0a89ea06/ol-19-01-1001-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14cc/6924186/1ef957590551/ol-19-01-1001-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14cc/6924186/4ef8545361ae/ol-19-01-1001-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14cc/6924186/468f2963c95b/ol-19-01-1001-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14cc/6924186/917aa3441e43/ol-19-01-1001-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14cc/6924186/929a0a89ea06/ol-19-01-1001-g04.jpg

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本文引用的文献

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Biomed Pharmacother. 2019 Jun;114:108662. doi: 10.1016/j.biopha.2019.108662. Epub 2019 Apr 15.
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microRNA-431 as a Chemosensitizer and Potentiator of Drug Activity in Adrenocortical Carcinoma.microRNA-431 作为促肾上腺皮质癌化疗增敏剂和药物活性增强剂。
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MiR-431 suppresses proliferation and metastasis of lung cancer via down-regulating DDX5.
通过直接靶向miR-431/LASP1轴鉴定环状RNA circ-CSPP1为结直肠癌的有效驱动因子。
Open Life Sci. 2021 May 29;16(1):523-536. doi: 10.1515/biol-2021-0053. eCollection 2021.
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Circ_0001367 inhibits glioma proliferation, migration and invasion by sponging miR-431 and thus regulating NRXN3.环状 RNA 0001367 通过海绵吸附 miR-431 从而调控 NRXN3 抑制神经纤维瘤病蛋白 3 表达抑制神经胶质瘤的增殖、迁移和侵袭。
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Long noncoding RNAs SET-binding factor 2-antisense RNA1 promotes cell growth through targeting miR-431-5p/CDK14 axis in human papillary thyroid cancer.长链非编码 RNA SET 结合因子 2-反义 RNA1 通过靶向 miR-431-5p/CDK14 轴促进人甲状腺乳头状癌细胞生长。
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Eur Rev Med Pharmacol Sci. 2019 Jan;23(2):699-707. doi: 10.26355/eurrev_201901_16883.
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Downregulation of miRNA-214 in cancer-associated fibroblasts contributes to migration and invasion of gastric cancer cells through targeting FGF9 and inducing EMT.肿瘤相关成纤维细胞中 miRNA-214 的下调通过靶向 FGF9 并诱导 EMT 促进胃癌细胞的迁移和侵袭。
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