Sun Liangzhi, Liu Ming, Luan Suxian, Shi Yulin, Wang Qiang
Department of Orthopedics, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China.
Department of Orthopedics, Hanting People's Hospital, Weifang, Shandong 261100, P.R. China.
Oncol Lett. 2019 Sep;18(3):2523-2529. doi: 10.3892/ol.2019.10530. Epub 2019 Jun 26.
Osteosarcoma (OS) mortality rate is increasing. Various microRNAs (miRNAs) have been investigated in the pathological process of OS except for miR-744. Hence, this research was designed to explore miR-744 function in OS. RT-qPCR and western blot analysis were used to quantify miR-744 and large tumor suppressor kinase 2 (LATS2) expression levels. The function of miR-744 was investigated using MTT and Transwell assays. Target gene of miR-744 was verified by dual-luciferase reporter assay. miR-744 expression was increased in OS, which was associated with worse clinical features and prognosis of OS patients. Importantly, miR-744 promoted cell viability and metastasis in OS. Furthermore, miR-744 induced Wnt/β-catenin pathway and epithelial-mesenchymal transition (EMT) in OS. In addition, miR-744 directly targeted LATS2 and blocked its expression in OS. Moreover, upregulation of LATS2 weakened the promotion of cell viability and metastasis induced by miR-744 in OS. In conclusion, miR-744 accelerated OS progression through restraining LATS2 and activating Wnt/β-catenin pathway and EMT.
骨肉瘤（OS）的死亡率正在上升。除了miR-744之外，多种微小RNA（miRNA）已在OS的病理过程中得到研究。因此，本研究旨在探索miR-744在OS中的功能。采用逆转录定量聚合酶链反应（RT-qPCR）和蛋白质免疫印迹分析来定量miR-744和大肿瘤抑制激酶2（LATS2）的表达水平。使用MTT法和Transwell实验来研究miR-744的功能。通过双荧光素酶报告基因实验验证miR-744的靶基因。miR-744在OS中的表达增加，这与OS患者较差的临床特征和预后相关。重要的是，miR-744促进了OS中的细胞活力和转移。此外，miR-744在OS中诱导Wnt/β-连环蛋白信号通路和上皮-间质转化（EMT）。另外，miR-744直接靶向LATS2并在OS中阻断其表达。而且，LATS2的上调减弱了miR-744在OS中诱导的细胞活力促进和转移作用。总之，miR-744通过抑制LATS2以及激活Wnt/β-连环蛋白信号通路和EMT来加速OS进展。
