Lehmann W D, Fischer R, Heinrich H C
Institute of Physiological Chemistry, University Hospital Eppendorf, Hamburg, Federal Republic of Germany.
Anal Biochem. 1988 Jul;172(1):151-9. doi: 10.1016/0003-2697(88)90424-1.
The methodology of precise isotope abundance determinations of erythrocyte iron by fast atom bombardment mass spectrometry and signal averaging is established. For the determination of the 54Fe/56Fe ratio a relative precision of 0.5% and an absolute precision of 0.03% is achieved. After oral loading with 54Fe-enriched samples in the range between 5 and 25 mg per subject, the 14-day erythrocyte incorporation of 54Fe has been determined in five individuals, namely, two adults, two children, and one infant. In the two adults, the oral dose of 54Fe was simultaneously labeled with a trace amount of carrier-free 59Fe. In these double-isotope loading tests, a good agreement was observed between the absorption data determined on the basis of whole body retention of 59Fe and on the basis of the 54Fe erythrocyte incorporation. The stable isotope methodology applied allows measurement of the iron absorption using highly enriched 54Fe at a dose of 25 mg for an adult or at a dose of 5 mg for infants of about 1 year of age.
建立了通过快原子轰击质谱法和信号平均法精确测定红细胞铁同位素丰度的方法。对于54Fe/56Fe比值的测定，实现了0.5%的相对精度和0.03%的绝对精度。在每位受试者口服5至25毫克范围内富含54Fe的样品后，测定了五名个体（两名成年人、两名儿童和一名婴儿）14天内红细胞对54Fe的摄取情况。在两名成年人中，口服的54Fe剂量同时用微量无载体59Fe进行标记。在这些双同位素加载试验中，基于59Fe全身滞留量测定的吸收数据与基于54Fe红细胞摄取量测定的吸收数据之间观察到良好的一致性。所应用的稳定同位素方法允许使用高富集的54Fe测量铁吸收，对于成年人剂量为25毫克，对于约1岁的婴儿剂量为5毫克。
