Huang Peipei, Guo Wenjie, Wang Yuhong, Xiong Yang, Ge Si, Gong Gaorui, Lin Qiaohong, Xu Zhen, Gui Jian-Fang, Mei Jie
School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, China; College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
Genomics. 2022 May;114(3):110380. doi: 10.1016/j.ygeno.2022.110380. Epub 2022 May 6.
Sexual size dimorphism has been widely observed in a large number of animals including fish species. Genome-wide association study (GWAS) is a powerful tool to dissect the genetic basis of complex traits, whereas the sex-differences in the genomics of animal complex traits have been ignored in the GWAS analysis. Yellow catfish (Pelteobagrus fulvidraco) is an important aquaculture fish in China with significant sexual size dimorphism. In this study, GWAS was conducted to identify candidate SNPs and genes related to body length (BL) and body weight (BW) in 125 female yellow catfish from a breeding population. In total, one BL-related SNP and three BW-related SNPs were identified to be significantly associated with the traits. Besides, one of these SNPs (Chr15:19195072) was shared in both the BW and BL traits in female yellow catfish, which was further validated in 185 male individuals and located on the exon of stat5b gene. Transgenic yellow catfish and zebrafish that expressed yellow catfish stat5b showed increased growth rate and reduction of sexual size dimorphism. These results not only reveal the genetic basis of growth trait and sexual size dimorphism in fish species, but also provide useful information for the marker-assisted breeding in yellow catfish.
性大小二态性在包括鱼类在内的大量动物中广泛存在。全基因组关联研究（GWAS）是剖析复杂性状遗传基础的有力工具，然而在GWAS分析中，动物复杂性状基因组学中的性别差异一直被忽视。黄颡鱼（Pelteobagrus fulvidraco）是中国重要的水产养殖鱼类，具有显著的性大小二态性。在本研究中，对来自一个育种群体的125尾雌性黄颡鱼进行了GWAS分析，以鉴定与体长（BL）和体重（BW）相关的候选单核苷酸多态性（SNP）和基因。总共鉴定出1个与BL相关的SNP和3个与BW相关的SNP与这些性状显著相关。此外，其中一个SNP（Chr15:19195072）在雌性黄颡鱼的BW和BL性状中均存在，在185尾雄性个体中进一步验证，并位于stat5b基因的外显子上。表达黄颡鱼stat5b的转基因黄颡鱼和斑马鱼生长速度加快，性大小二态性降低。这些结果不仅揭示了鱼类生长性状和性大小二态性的遗传基础，也为黄颡鱼的分子标记辅助育种提供了有用信息。
