Piatyszek M A, Jarmolowski A, Augustyniak J
A Mickiewicz University, Department of Bipolymer Biochemistry, Poznan, Poland.
Anal Biochem. 1988 Aug 1;172(2):356-9. doi: 10.1016/0003-2697(88)90455-1.
Iodo-Gen (1,3,4,6-tetrachloro-3a,6a-diphenylglycoluril), widely used as an oxidizing agent for iodination of proteins, can also be used for iodination of nucleic acids. Optimal conditions were determined for efficient labeling of RNA and DNA with 125I. The proposed procedure for radioiodination of nucleic acids is more beneficial than the methods utilizing TlCl3 because of the milder reaction conditions, the simplicity and completeness of separation of reaction products from the oxidizing agents, and the absence of a toxic catalyst. Using the standard procedure for Iodo-Gen-mediated iodination a specific radioactivity of up to 1.3 X 10(9) dpm/micrograms RNA can be achieved. The proposed procedure is also suitable for radioiodination of DNA.
碘甘醚（1,3,4,6-四氯-3a,6a-二苯基甘脲）被广泛用作蛋白质碘化的氧化剂，也可用于核酸的碘化。已确定了用125I高效标记RNA和DNA的最佳条件。所提出的核酸放射性碘化方法比使用TlCl3的方法更具优势，因为反应条件更温和、反应产物与氧化剂的分离简单且完全，并且不存在有毒催化剂。使用碘甘醚介导碘化的标准程序，可实现高达1.3×10(9) dpm/微克RNA的比放射性。所提出的程序也适用于DNA的放射性碘化。
