School of Food and Biological Engineering Jiangsu University, Xuefu Road 301, Zhenjiang, 212013, People's Republic of China.
Mikrochim Acta. 2020 Jan 2;187(1):85. doi: 10.1007/s00604-019-4030-4.
A fluorescent nanoprobe for Pb(II) has been developed by employing aptamer-functionalized upconversion nanoparticles (UCNPs) and magnetic FeO-modified (MNPs) gold nanoparticles (GNPs). First, aptamer-functionalized UCNPs and aptamer-functionalized magnetic GNPs were synthesized to obtained the fluorescent nanoprobe. The particles were combined by adding a complementary ssDNA. In the absence of Pb(II), the UCNPs, MNPs and GNPs are linked via complementary base pairing. This led to a decrease in the green upconversion fluorescence peaking at 547 nm (under 980 nm excitation). In the presence of Pb(II), the dsDNA between UCNPs and MNPs-GNPs is cleaved, and fluorescence recovers. This effect allows Pb(II) to be quantified, with a wide working range of 25-1400 nM and a lower detection limit of 5.7 nM. The nanoprobe gave satisfactory results when analyzing Pb(II) in tea and waste water. Graphical abstractSchematic representation of fluorescent nanoprobe based on fluorescence resonance energy transfer (FRET) between upconversion nanoparticles (UCNPs) and gold nanoparticles (GNPs)-FeO magnetic nanoparticles (MNPs) for detection of Pb.
一种基于上转换纳米粒子（UCNPs）和金纳米粒子（GNPs）-FeO 磁性纳米粒子（MNPs）之间荧光共振能量转移（FRET）的荧光纳米探针被开发出来用于检测 Pb(II)。首先，合成了适配体功能化的上转换纳米粒子（UCNPs）和适配体功能化的磁性金纳米粒子（GNPs），以获得荧光纳米探针。通过添加互补的单链 DNA 将粒子结合在一起。在没有 Pb(II) 的情况下，UCNPs、MNPs 和 GNPs 通过互补碱基配对连接在一起。这导致在 547nm 处的绿色上转换荧光峰（在 980nm 激发下）减少。在存在 Pb(II) 的情况下，UCNPs 和 MNPs-GNPs 之间的双链 DNA 被切割，荧光恢复。这种效应允许定量检测 Pb(II)，其工作范围很宽，为 25-1400nM，检测下限为 5.7nM。该纳米探针在分析茶叶和废水中的 Pb(II)时取得了令人满意的结果。示意图荧光纳米探针的示意图，基于上转换纳米粒子（UCNPs）和金纳米粒子（GNPs）-FeO 磁性纳米粒子（MNPs）之间的荧光共振能量转移（FRET），用于检测 Pb。
