National Engineering Research Center for Nanomedicine, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, China.
Nat Commun. 2020 Jan 3;11(1):81. doi: 10.1038/s41467-019-13784-0.
Live-cell Raman imaging based on bioorthogonal Raman probes with distinct signals in the cellular Raman-silent region (1800-2800 cm) has attracted great interest in recent years. We report here a class of water-soluble and biocompatible polydiacetylenes with intrinsic ultrastrong alkyne Raman signals that locate in this region for organelle-targeting live-cell Raman imaging. Using a host-guest topochemical polymerization strategy, we have synthesized a water-soluble and functionalizable master polydiacetylene, namely poly(deca-4,6-diynedioic acid) (PDDA), which possesses significantly enhanced (up to ~10 fold) alkyne vibration compared to conventional alkyne Raman probes. In addition, PDDA can be used as a general platform for multi-functional ultrastrong Raman probes. We achieve high quality live-cell stimulated Raman scattering imaging on the basis of modified PDDA. The polydiacetylene-based Raman probes represent ultrastrong intrinsic Raman imaging agents in the Raman-silent region (without any Raman enhancer), and the flexible functionalization of this material holds great promise for its potential diverse applications.
近年来，基于在细胞拉曼静默区（1800-2800cm）中具有独特信号的生物正交拉曼探针的活细胞拉曼成像引起了极大的兴趣。我们在这里报道了一类具有内在超强度炔烃拉曼信号的水溶性和生物相容性聚二乙炔，可用于细胞器靶向活细胞拉曼成像。我们使用主客体拓扑聚合策略，合成了一种水溶性和可功能化的主聚二乙炔，即聚（癸-4,6-二炔二酸）（PDDA），与传统的炔烃拉曼探针相比，其炔振动显著增强（高达~10 倍）。此外，PDDA可用作多功能超强度拉曼探针的通用平台。我们在修饰后的 PDDA 基础上实现了高质量的活细胞受激拉曼散射成像。基于聚二乙炔的拉曼探针代表了拉曼静默区（无需任何拉曼增强剂）中的超强度本征拉曼成像剂，该材料的灵活功能化有望为其潜在的多种应用提供广阔的前景。
