Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.
Division of Bone Diseases, Department of Internal Medicine Specialties, Faculty of Medicine, Geneva University Hospital, Geneva, Switzerland.
Calcif Tissue Int. 2020 Apr;106(4):431-443. doi: 10.1007/s00223-019-00652-8. Epub 2020 Jan 4.
We explored age- and strain-related differences in bone microstructure and body composition in male C57BL/6J, DBA/2JRj and C3H/J mice. Bone microstructure of the femur, tibia and L4 was assessed by μCT at the age of 8, 16 and 24 weeks. The weight of several muscles and fat depots were measured at the same time points. At all timepoints, C3H/J mice had the thickest cortices followed by DBA/2JRj and C57BL/6J mice. Nevertheless, C57BL/6J mice had higher Tb.BV/TV and Tb.N, and lower Tb.Sp than DBA/2JRj and C3H/J mice at least at 24 weeks of age. Skeletal development patterns differed among strains. C57BL/6J and DBA/2JRj mice, but not C3H/J mice, experienced significant increases in the sum of the masses of 6 individual muscles by 24 weeks of age. In C57BL/6J and DBA/2JRj mice, the mass of selected fat depots reached highest values at 24 weeks, whist, in C3H/J mice, the highest values of fat depots masses were achieved at 16 weeks. Early strain differences in muscle and fat masses were largely diminished by 24 weeks of age. C3H/J and C57BL/6J mice displayed the most favorable cortical and trabecular bone parameters, respectively. Strain differences in body composition were less overt than strain specificity in bone microstructure, however, they possibly influenced aspects of skeletal development.
我们研究了雄性 C57BL/6J、DBA/2JRj 和 C3H/J 小鼠的年龄和品系相关的骨微结构和身体成分差异。在 8、16 和 24 周龄时，通过 μCT 评估股骨、胫骨和 L4 的骨微结构。同时测量了几个肌肉和脂肪组织的重量。在所有时间点，C3H/J 小鼠的皮质最厚，其次是 DBA/2JRj 和 C57BL/6J 小鼠。然而，至少在 24 周龄时，C57BL/6J 小鼠的 Tb.BV/TV 和 Tb.N 较高，Tb.Sp 较低，而 DBA/2JRj 和 C3H/J 小鼠则较低。骨骼发育模式在不同品系之间存在差异。C57BL/6J 和 DBA/2JRj 小鼠，但不是 C3H/J 小鼠，在 24 周龄时经历了 6 块肌肉总质量的显著增加。在 C57BL/6J 和 DBA/2JRj 小鼠中，所选脂肪组织的质量在 24 周时达到最高值，而在 C3H/J 小鼠中，脂肪组织的质量在 16 周时达到最高值。到 24 周龄时，肌肉和脂肪组织质量的早期品系差异大大减少。C3H/J 和 C57BL/6J 小鼠分别表现出最有利的皮质和小梁骨参数。身体成分的品系差异不如骨微结构的品系特异性明显，但它们可能影响骨骼发育的某些方面。
