Quantitative analysis of lead in cows and buffaloes for health assessment.

The present study aimed to investigate the concentration of highly toxic heavy metal, lead (Pb), in cows and buffaloes of Sargodha district, Punjab, Pakistan. Samples of three major organs (spleen, lungs, bones) from five localities were obtained from two age groups (< 2 years, > 2 years) of each animal. With the confidence level of 95%, the investigated results demonstrated that lead concentration ranged from 0.28 to 1.12 mg kg and 0.31 to 0.83 mg kg in cows and buffaloes respectively. Out of the three tested organs, spleen contained the highest level of Pb (0.64-0.83 mg/kg) followed by lungs (0.28-1.12 mg/kg) and bones (0.36-0.87 mg/kg). Furthermore, a significant increase of Pb was observed in older animals. It was demonstrated that both age groups had Pb accumulation higher than the standard limit of 0.1 mg kg. Serving as bioindicators, the presence of toxic levels of lead in the animals indicated environmental pollution and possible risks for public health. High lead concentration in dietary commodities from Sargodha district calls for the sound management of hazardous waste. High levels of this toxic metal also indicate the possible health risks to human population of the areas in Sargodha. It is highly needed to address this metal contamination and its probable entry route into the food chain of the domesticated animals. This must be further investigated to prevent animals from lead exposure.