β-贝壳杉烯-15-烯-17-酸对阿尔茨海默病淀粉样β肽诱导的氧化凋亡的神经保护作用。

Neuroprotective Effect of -Kaur-15-en-17-al-18-oic Acid on Amyloid Beta Peptide-Induced Oxidative Apoptosis in Alzheimer's Disease.

机构信息

Marine College, Shandong University, Weihai 264209, Shandong, China.

School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China.

出版信息

Molecules. 2019 Dec 29;25(1):142. doi: 10.3390/molecules25010142.

-Kaur-15-en-17-al-18-oic acid, extracted from the Chinese well known folk herb , performed a significantly neuroprotective effect on amyloid beta peptide 25-35 (Aβ)-induced SH-SY5Y cells neurotoxicity in Alzheimer's disease. The results demonstrated that this compound maintained oxidative stress balance, reduced levels of reactive oxygen species (ROS), malondialdehyde (MDA), and improved contents of glutathione (GSH) and superoxide dismutase (SOD) without obvious cytotoxicity. This compound also obviously relieved oxidative stress-induced apoptosis associated with p53 and nuclear factor κB (NF-κB) pathways accompanied by upregulating B-cell lymphoma-2 (bcl-2) and downregulating p53, nuclear factor κB (NF-κB), Bax, Cleaved-caspase 3, and Cytochrome C protein expressions further. Briefly, -kaur-15-en-17-al-18-oic acid protected cells from oxidative apoptosis associated with p53 and NF-κB pathways.

从中国著名的民间草药中提取的-kaur-15-en-17-al-18-oic 酸对阿尔茨海默病中淀粉样β肽 25-35（Aβ）诱导的 SH-SY5Y 细胞神经毒性表现出显著的神经保护作用。结果表明，该化合物维持氧化应激平衡，降低活性氧（ROS）、丙二醛（MDA）水平，提高谷胱甘肽（GSH）和超氧化物歧化酶（SOD）含量，同时无明显细胞毒性。该化合物还明显缓解了与 p53 和核因子 κB（NF-κB）通路相关的氧化应激诱导的细胞凋亡，同时上调 B 细胞淋巴瘤-2（bcl-2）并下调 p53、核因子 κB（NF-κB）、Bax、Cleaved-caspase 3 和细胞色素 C 蛋白的表达。简而言之，-kaur-15-en-17-al-18-oic 酸可防止细胞发生与 p53 和 NF-κB 通路相关的氧化凋亡。