Yamaguchi F, Hattori H, Wagai N, Tsukada W
Research Institute, Daiichi Seiyaku Co., Ltd., Tokyo, Japan.
Arzneimittelforschung. 1988 Jul;38(7A):1034-7.
Antigenicity study of N2-[(N-acetylmuramoyl)-L-alanyl-D-isoglutaminyl]-N6-stearoyl-L-lysi++ + ne (MDP-Lys(L18), muroctasin) was carried out in mice, guinea pigs and rabbits with passive cutaneous anaphylaxis (PCA), systemic anaphylaxis (SA), Arthus and delayed skin reaction and enzyme-linked immunosorbent assay (ELISA). Mice were sensitized intraperitoneally, twice at 3-week intervals, with MDP-Lys(L18) (40, 400 or 4000 micrograms/kg) or MDP-Lys(L18)-ovalbumin (OA) conjugate (500 micrograms/kg) in alumina gel. No IgE antibodies to MDP-Lys(L18) were detected in the sera obtained from the sensitized mice by 24-h PCA in rat. Guinea pigs were sensitized subcutaneously with MDP-Lys(L18) (4, 40 or 400 micrograms/kg) or MDP-Lys(L18)-OA (2 mg/kg) emulsified in Freund's complete and incomplete adjuvant, 3 times at 2-week intervals. No SA was observed in the sensitized animals after the intravenous injection of MDP-Lys(L18) (1 mg/kg). Rabbits were sensitized subcutaneously with MDP-Lys(L18) (2 or 20 micrograms/kg) or MDP-Lys(L18)-OA (2 mg/kg) emulsified in Freund's incomplete adjuvant, 3 times at 2-week intervals. Neither Arthus nor delayed skin reaction was observed in the sensitized animals after the intradermal injection of MDP-Lys(L18) (2 micrograms/site). Although antibodies to MDP-Lys(L18) were not detected in MDP-Lys(L18) sensitized animals, the antibodies were detected in two out of 9 guinea pigs and all of the rabbits in the MDP-Lys(L18)-OA sensitized groups by 4-h PCA after the intravenous injection of MDP-Lys(L18) (1 mg/kg). These results suggest that MDP-Lys(L18) may possess antigenic potential by PCA reaction in guinea pigs.
采用被动皮肤过敏反应（PCA）、全身过敏反应（SA）、阿瑟斯反应和迟发性皮肤反应以及酶联免疫吸附测定（ELISA），在小鼠、豚鼠和兔子身上开展了N2-[(N-乙酰胞壁酰)-L-丙氨酰-D-异谷氨酰胺基]-N6-硬脂酰-L-赖氨酸（MDP-Lys(L18)，胞壁酰三肽赖氨酸）的抗原性研究。给小鼠腹腔注射致敏，每隔3周注射两次，注射用的是氧化铝凝胶中的MDP-Lys(L18)（40、400或4000微克/千克）或MDP-Lys(L18)-卵清蛋白（OA）偶联物（500微克/千克）。在通过大鼠24小时PCA从致敏小鼠获得的血清中，未检测到针对MDP-Lys(L18)的IgE抗体。给豚鼠皮下注射用弗氏完全佐剂和不完全佐剂乳化的MDP-Lys(L18)（4、40或400微克/千克）或MDP-Lys(L18)-OA（2毫克/千克），每隔2周注射3次。给致敏动物静脉注射MDP-Lys(L18)（1毫克/千克）后，未观察到全身过敏反应。给兔子皮下注射用弗氏不完全佐剂乳化的MDP-Lys(L18)（2或20微克/千克）或MDP-Lys(L18)-OA（2毫克/千克），每隔2周注射3次。给致敏动物皮内注射MDP-Lys(L18)（2微克/部位）后，未观察到阿瑟斯反应和迟发性皮肤反应。虽然在MDP-Lys(L18)致敏动物中未检测到针对MDP-Lys(L18)的抗体，但在静脉注射MDP-Lys(L18)（1毫克/千克）后4小时PCA检测中，MDP-Lys(L18)-OA致敏组的9只豚鼠中有2只以及所有兔子中检测到了抗体。这些结果表明，MDP-Lys(L18)在豚鼠中可能通过PCA反应具有抗原潜力。
