Kim D N, Scott R F, Schmee J, Thomas W A
Department of Pathology, Albany Medical College, NY 12208.
Atherosclerosis. 1988 Oct;73(2-3):247-57. doi: 10.1016/0021-9150(88)90048-2.
This study shows that frank endothelial denudation, as measured by scanning electron microscopy, is associated with coronary artery atherosclerotic lesions in swine fed hyperlipidemic diets for 9 or 18 months. The largest areas of endothelial cell denudation were found to be associated with the most advanced atherosclerotic lesions, and lesser areas of denudation were present in less severely atherosclerotic arteries. Overall, the study suggests that frank endothelial denudation in swine coronaries occurs secondarily to advanced lesion development, and is proportionate to the amount of lesion present. In contrast attachment of monocytes to the endothelial surface was not proportionate to the amount of lesion present. While the number of attached monocytes after 9 or 18 months of a hyperlipidemic diet was much greater than in the mash-fed swine, there was no difference between the two hyperlipidemic groups. In both hyperlipidemic groups, the attached monocytes were concentrated over lesion areas. By scanning electron microscopy, most attached monocytes in these perfused coronary arteries showed a slender foot process extending into a gap between endothelial cells. Endothelial cell turnover, as measured by tritiated thymidine labelling indices, increased with the amount of coronary artery atherosclerosis. However, it did not become significantly greater than in mash-fed swine until advanced lesions developed.
本研究表明，通过扫描电子显微镜测量，明显的内皮剥脱与高脂饮食喂养9个月或18个月的猪冠状动脉粥样硬化病变相关。内皮细胞剥脱面积最大的区域与最严重的动脉粥样硬化病变相关，而剥脱面积较小的区域则存在于动脉粥样硬化程度较轻的动脉中。总体而言，该研究表明猪冠状动脉明显的内皮剥脱是在晚期病变发展之后发生的，并且与病变的数量成正比。相比之下，单核细胞在内皮表面的附着与病变的数量不成正比。虽然高脂饮食9个月或18个月后附着的单核细胞数量比喂谷物饲料的猪多得多，但两个高脂组之间没有差异。在两个高脂组中，附着的单核细胞都集中在病变区域。通过扫描电子显微镜观察，这些灌注冠状动脉中大多数附着的单核细胞显示出细长的足突延伸到内皮细胞之间的间隙中。通过氚标记胸腺嘧啶核苷标记指数测量的内皮细胞更新率随着冠状动脉粥样硬化的程度而增加。然而，直到晚期病变发展，它才显著高于喂谷物饲料的猪。
