用于载脂蛋白E表型分析的半自动等电聚焦方法的评估
Evaluation of a semi-automatic isoelectric focusing method for apolipoprotein E phenotyping.
作者信息
Bittar Randa, Carrié Alain, Nouadje Georges, Cherfils Corinne, Fesel-Fouquier Valérie, Barbot-Trystram Laurence, Giral Philippe, Bonnefont-Rousselot Dominique
机构信息
Service de Biochimie Métabolique, AP-HP.Sorbonne Université, F-75013, Paris, France.
Sorbonne Université, UMR_S1166 ICAN, F-75013, Paris, France.
出版信息
Pract Lab Med. 2019 Dec 17;18:e00150. doi: 10.1016/j.plabm.2019.e00150. eCollection 2020 Jan.
A qualitative, semi-automatized method for apolipoprotein E (apoE) phenotyping by isoelectric focusing method has been evaluated on 40 serum samples from patients previously genotyped for apoE, especially as regards concordance with genotyping, but also repeatability and reproducibility of the method, and sample storage. Total concordance with genotyping and good precision criteria, together with its practicability and requirement of a little sample volume, lead to conclude to the usefulness of this method to help clinicians in the diagnosis of dyslipidemic and neurodegenerative diseases.
一种通过等电聚焦法对载脂蛋白E（apoE）进行表型分析的定性、半自动方法，已在40份来自先前已进行apoE基因分型的患者的血清样本上进行了评估，特别是在与基因分型的一致性方面，同时也评估了该方法的重复性、再现性以及样本储存情况。与基因分型的完全一致性、良好的精密度标准，以及其实用性和对少量样本量的要求，使得我们得出结论：该方法有助于临床医生诊断血脂异常和神经退行性疾病。
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