This study investigated the effect of high-intensity cycling re-warm up (RW) within a very short time-frame on the subsequent intermittent sprint performance. Twelve active males completed three trials in random order: control (CON); 3-min RW at 30% of maximal oxygen uptake (VO) (RW30); and 1-min RW at 90% of VO (RW90). During the experimental trials, participants performed 40-min intermittent cycling exercise followed by 15-min rest. During the rest period, participants completed CON, RW30, or RW90. After the rest period, participants performed the Cycling Intermittent-Sprint Protocol (CISP), which consisted of 10-s rest, 5-s maximal sprint, and 105-s active recovery with the cycles repeated over 10 min. The mean work during sprint for the CISP was significantly higher in both RW trials than in the CON trial (mean±standard deviation; CON: 3539±698 J; RW30: 3724±720 J; RW90: 3739±736 J; <0.05). The mean electromyogram amplitude during the sprint for the CISP was higher in the RW30 trial than in the CON trial; however, there was no significant difference between the two trials (=0.06). The mean median frequency during sprint for the CISP was significantly higher in the RW90 trial than in the other trials (<0.05). Rectal temperature did not differ among the three trials. Oxygenated haemoglobin during the initial 30 s of the CISP was significantly higher in the RW90 trial than in the CON trial (<0.05). Compared with seated rest, RW, irrespective of whether it comprised 1-min at 90% of VO or 3-min at 30% of VO, increased the subsequent intermittent sprint performance.