一种用于检测溶菌碱和胭脂碱脱氢酶活性的快速微量方法。
A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities.
作者信息
Otten L A, Schilperoort R A
出版信息
Biochim Biophys Acta. 1978 Dec 8;527(2):497-500. doi: 10.1016/0005-2744(78)90363-7.
PMID:31918
Abstract
A rapid and sensitive method has been developed to determine lysopine dehydrogenase (EC 1.5.1-) and nopaline dehydrogenase activities in crown gall tumour tissues. By this method, enzyme activities as low as 0.2 micrometerol octopine or nopaline per h per g fresh weight tumour tissue can still be detected. In non-infected young pea seedlings, no lysopine dehydrogenase activity was detected.
摘要
已开发出一种快速灵敏的方法来测定冠瘿瘤组织中的赖氨酸脱氢酶（EC 1.5.1-）和胭脂碱脱氢酶活性。通过这种方法，每小时每克鲜重肿瘤组织中低至0.2微摩尔章鱼碱或胭脂碱的酶活性仍可被检测到。在未感染的豌豆幼苗中，未检测到赖氨酸脱氢酶活性。
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