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一种用于检测溶菌碱和胭脂碱脱氢酶活性的快速微量方法。

A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities.

作者信息

Otten L A, Schilperoort R A

出版信息

Biochim Biophys Acta. 1978 Dec 8;527(2):497-500. doi: 10.1016/0005-2744(78)90363-7.

DOI:10.1016/0005-2744(78)90363-7
PMID:31918
Abstract

A rapid and sensitive method has been developed to determine lysopine dehydrogenase (EC 1.5.1-) and nopaline dehydrogenase activities in crown gall tumour tissues. By this method, enzyme activities as low as 0.2 micrometerol octopine or nopaline per h per g fresh weight tumour tissue can still be detected. In non-infected young pea seedlings, no lysopine dehydrogenase activity was detected.

摘要

已开发出一种快速灵敏的方法来测定冠瘿瘤组织中的赖氨酸脱氢酶（EC 1.5.1-）和胭脂碱脱氢酶活性。通过这种方法，每小时每克鲜重肿瘤组织中低至0.2微摩尔章鱼碱或胭脂碱的酶活性仍可被检测到。在未感染的豌豆幼苗中，未检测到赖氨酸脱氢酶活性。

相似文献

1
A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities.一种用于检测溶菌碱和胭脂碱脱氢酶活性的快速微量方法。
Biochim Biophys Acta. 1978 Dec 8;527(2):497-500. doi: 10.1016/0005-2744(78)90363-7.
2
Octopine and nopaline metabolism in Agrobacterium tumefaciens and crown gall tumor cells: role of plasmid genes.根癌土壤杆菌和冠瘿瘤细胞中的章鱼碱和胭脂碱代谢：质粒基因的作用
J Bacteriol. 1977 Jan;129(1):101-7. doi: 10.1128/jb.129.1.101-107.1977.
3
Properties of D(+)-lysopine dehydrogenase from crown gall tumour tissue.来自冠瘿瘤组织的D(+)-溶菌碱脱氢酶的特性
Biochim Biophys Acta. 1977 Dec 8;485(2):268-77. doi: 10.1016/0005-2744(77)90163-2.
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Purification and characterization of the crown gall specific enzyme nopaline synthase.冠瘿特异性酶胭脂碱合酶的纯化与特性分析
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Promotion of crown-gall tumor growth by lysopine, octopine, nopaline, and carnosine.农杆碱、章鱼碱、胭脂碱和肌肽对冠瘿瘤生长的促进作用。
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Comparison of octopine, histopine, lysopine, and octopinic acid synthesizing activities in sunflower crown gall tissues.向日葵冠瘿组织中章鱼碱、组氨酸章鱼碱、赖氨酸章鱼碱和章鱼氨酸合成活性的比较。
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引用本文的文献

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Plant Cell Rep. 1997 Mar;16(6):389-392. doi: 10.1007/BF01146779.
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Enhancement of reproductive heat tolerance in plants.提高植物的繁殖耐热性。
PLoS One. 2015 Apr 7;10(4):e0122933. doi: 10.1371/journal.pone.0122933. eCollection 2015.
3
Clonal analysis of heterogeneous crown gall tumor tissues induced by wild-type and shooter mutant strains ofAgrobacterium tumefaciens-expression of T-DNA genes.
利用野生型和突变型根癌农杆菌诱导的异质性冠瘿瘤组织的克隆分析——T-DNA 基因的表达。
Plant Mol Biol. 1983 Nov;2(6):321-33. doi: 10.1007/BF01578594.
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Double infection of tobacco plants by two complementing octopine T-region mutants of Agrobacterium tumefaciens.烟草植株被 2 株互补的根癌农杆菌 octopine T 区突变体的双重感染。
Plant Mol Biol. 1982 Sep;1(3):217-26. doi: 10.1007/BF00021033.
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The lysopinedehydrogenase gene used as a marker for the selection of octopine crown gall cells.作为选择胭脂碱冠瘿细胞的标记物的赖氨酸脱氢酶基因。
Plant Mol Biol. 1982 Jun;1(2):133-42. doi: 10.1007/BF00024976.
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Plant Mol Biol. 1984 Nov;3(6):371-8. doi: 10.1007/BF00033384.
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