J.A. Cohen Institute for Radiopathology and Radiation Protection, Leiden.
Plant Mol Biol. 1982 Jun;1(2):133-42. doi: 10.1007/BF00024976.
Plant cells transformed into octopine-synthesizing tumour cells by the bacterium Agrobacterium tumefaciens survive when cultured in the presence of homo-arginine (HA), whereas both normal plant cells and nopaline producing plant tumour cells do not. Survival of octopine crown gall cells is due to the activity of the enzyme lysopinedehydrogenase (LpDH) in these cells, which converts toxic homo-arginine into non-toxic homo-octopine. The selective toxicity of homo-arginine for normal cells can be applied for the enrichment of octopine Ti plasmid transformed plant cells vs normal plant cells in mixed cultures.
当用农杆菌转化的细菌 Agrobacterium tumefaciens 将植物细胞转化为合成章鱼碱的肿瘤细胞时，这些细胞在存在同型精氨酸 (HA) 的情况下可以存活，而正常植物细胞和胭脂碱产生的植物肿瘤细胞则不能。章鱼碱冠瘿瘤细胞的存活是由于这些细胞中赖氨酸脱氢酶 (LpDH) 的活性，该酶将有毒的同型精氨酸转化为无毒的同型章鱼碱。同型精氨酸对正常细胞的选择性毒性可用于富集混合培养中章鱼碱 Ti 质粒转化的植物细胞与正常植物细胞。
