Dukhanin A S
Farmakol Toksikol. 1988 Jul-Aug;51(4):71-2.
Using the fluorescent dye, quin-2, it was shown that the addition of cortisol, dexamethasone (10(-5)-10(-4) M) to the suspension of rat thymocytes results in 1.5-3-fold increase of free Ca2+ concentration in the cytoplasm (intracellular Ca2+). The hormone-stimulated increase of intracellular Ca2+ fails to occur if extracellular Ca2+ concentration is sharply reduced by using calcium-free buffer.
使用荧光染料喹啉-2表明,向大鼠胸腺细胞悬液中添加皮质醇、地塞米松(10^(-5)-10^(-4)M)会导致细胞质(细胞内Ca2+)中游离Ca2+浓度增加1.5至3倍。如果使用无钙缓冲液急剧降低细胞外Ca2+浓度,则不会出现激素刺激引起的细胞内Ca2+增加。