Rossi A G, Thomas M J, O'Flaherty J T
Department of Medicine, Wake Forest University Medical Center, Winston-Salem, NC 27103.
FEBS Lett. 1988 Nov 21;240(1-2):163-6. doi: 10.1016/0014-5793(88)80360-0.
5(S)-Hydroxyicosatetraenoate stimulates human polymorphonuclear neutrophils (PMNs) to raise their cytosolic calcium. It also potentiates the cells' degranulation responses to platelet-activating factor and diacylglycerols. We synthesized 5(R)-hydroxyicosatetraenoate and found it to be 20-100-fold weaker than the natural isomer in these assays. Thus, the arachidonic acid metabolite activates PMNs by a stereospecific possibly receptor-mediated mechanism.
5(S)-羟基二十碳四烯酸刺激人多形核中性粒细胞（PMNs）升高其胞质钙。它还增强细胞对血小板活化因子和二酰基甘油的脱颗粒反应。我们合成了5(R)-羟基二十碳四烯酸，发现在这些测定中它比天然异构体弱20至100倍。因此，花生四烯酸代谢物通过一种立体特异性的、可能是受体介导的机制激活PMNs。
