O'Flaherty J T, Nishihira J
Department of Medicine, Wake Forest University Medical Center, Winston-Salem, North Carolina 27103.
Biochem Biophys Res Commun. 1987 Oct 29;148(2):575-81. doi: 10.1016/0006-291x(87)90915-6.
5-Hydroxyeicosatetraenoate (5-HETE) stimulated human neutrophils to elevate their cytosolic Ca2+. It also enhanced the actions of phorbol myristate acetate and a diacylglycerol in stimulating these cells to: a) translocate protein kinase C from cytosol to membranes; b) generate superoxide anion; and c) release granule-bound enzymes. 5,20-DiHETE and 15-HETE possessed little or none of these bioactions. 5-HETE may influence neutrophil function by elevating cytosolic Ca2+ and thereby promoting the mobilization of protein kinase C.
5-羟基二十碳四烯酸（5-HETE）刺激人类中性粒细胞，使其胞质钙离子浓度升高。它还增强了佛波醇肉豆蔻酸酯乙酸盐和二酰甘油在刺激这些细胞方面的作用，具体表现为：a）使蛋白激酶C从胞质转移至细胞膜；b）产生超氧阴离子；c）释放颗粒结合酶。5,20-二羟基二十碳四烯酸（5,20-DiHETE）和15-羟基二十碳四烯酸（15-HETE）几乎没有或完全没有这些生物活性。5-HETE可能通过升高胞质钙离子浓度，进而促进蛋白激酶C的动员来影响中性粒细胞功能。
