Consortium of Probiotics Attenuates Colonization of .

infection (CDI) is increasing morbidity and mortality rates globally. Fecal microbiota transplantation (FMT), an effective therapy for eliminating (), cannot be used extensive due to a range of challenges. Probiotics thus constitutes a promising alternative therapy. In our study, we evaluated the effect of consortium of probiotics including five strains and two strains on the colonization of toxigenic BI/NAP1/027 in a mouse model. The results of 16S rRNA sequencing and targeted metabolomics showed the consortium of probiotics effectively decreased the colonization of , changed the α- and β-diversity of the gut microbiota, decreased the primary bile acids, and increased the secondary bile acids. Spearman's correlation showed that some of the OTUs such as , , . were positively correlated with numbers and the primary bile acids, and negatively correlated with the secondary bile acids. However, some of the OTUs, such as , , and Rikenellaceae, were negatively correlated with copies and the primary bile acids, and positively correlated with the secondary bile acids. In summary, the consortium of probiotics effectively decreases the colonization of , probably via alteration of gut microbiota and bile acids. Our probiotics mixture thus offers a promising FMT alternative.