Dai Yuanyuan, Chen Li, Chang Wenjiao, Lu Huaiwei, Cui Peng, Ma Xiaoling
Department of Clinical Laboratory, First Affiliated Hospital of University of Science and Technology of China, Hefei, China.
Infectious Disease Research and Development, Beijing Genomics Institute-Shanghai, Shanghai, China.
Front Public Health. 2019 Dec 17;7:379. doi: 10.3389/fpubh.2019.00379. eCollection 2019.
is a zoonotic pathogen that can cause severe infections such as meningitis and septicemia in both swine and humans. Rapid and accurate identification of the causative agent is very important for guiding clinical choices in administering countermeasures. Here, we report a case of fatal infection in a patient who worked as a butcher in China. The 59-year-old man, who had previously undergone splenectomy, injured his finger while processing pork and developed severe sepsis. While blood cultures were negative following antibiotic treatment, was determined to be the causative agent by metagenomic next-generation sequencing (mNGS) and Sanger sequencing. Identification of etiological agents using techniques such as blood culture prior to antibiotic treatment is very important. mNGS may represent a useful method for diagnosis of infectious diseases, especially post-antibiotic treatment.
是一种人畜共患病原体，可在猪和人类中引起严重感染，如脑膜炎和败血症。快速准确地鉴定病原体对于指导临床采取应对措施的选择非常重要。在此，我们报告一例在中国从事屠夫工作的患者发生的致命感染病例。这名59岁的男子此前接受过脾切除术，在处理猪肉时手指受伤并发展为严重败血症。虽然抗生素治疗后血培养结果为阴性，但通过宏基因组下一代测序（mNGS）和桑格测序确定其为病原体。在抗生素治疗前使用血培养等技术鉴定病原体非常重要。mNGS可能是诊断传染病的一种有用方法，尤其是在抗生素治疗后。
