Sardari Roya R R, Sutiono Samuel, Azeem Hafiz Abdul, Galbe Mats, Larsson Mats, Turner Charlotta, Nordberg Karlsson Eva
Division of Biotechnology, Department of Chemistry, Lund University, Lund, Sweden.
Chair of Chemistry of Biogenic Resources, Technical University of Munich, Munich, Germany.
Front Bioeng Biotechnol. 2019 Dec 13;7:413. doi: 10.3389/fbioe.2019.00413. eCollection 2019.
In line with the need to better utilize agricultural resources, and valorize underutilized fractions, we have developed protocols to increase the use of wheat bran, to improve utilization of this resource to additional products. Here, we report sequential processing for extraction of starch, lipids, and proteins from wheat brans with two different particle sizes leaving a rest-material enriched in dietary fiber. Mild water-based extraction of starch resulted in maximum 81.7 ± 0.67% yield. Supercritical fluid extraction of lipids by CO resulted in 55.2 ± 2.4% yield. This was lower than the corresponding yield using Soxhlet extraction, which was used as a reference method, but allowed a continued extraction sequence without denaturation of the proteins remaining in the raw-material. Alkaline extraction of non-degraded proteins resulted in a yield corresponding to one third of the total protein in the material, which was improved to reach 62 ± 8% by a combination of wheat bran enzymes activation followed by Osborne fractionation. The remaining proteins were extracted in degraded form, resulting in maximum 91.6 ± 1.6% yield of the total proteins content. The remaining material in both fine and coarse bran had a fiber content that on average corresponded to 73 ± 3%. The current work allows separation of several compounds, which is enabling valorization of the bran raw-material into several products.
为了更好地利用农业资源并使未充分利用的部分增值，我们开发了一些方案来增加麦麸的使用量，以提高这种资源在其他产品中的利用率。在此，我们报告了从两种不同粒径的麦麸中依次提取淀粉、脂质和蛋白质的方法，剩余的富含膳食纤维的物料。温和的水基淀粉提取法的最大产率为81.7±0.67%。用CO2进行超临界流体脂质提取的产率为55.2±2.4%。这低于用作参考方法的索氏提取法的相应产率，但允许连续提取，且不会使原料中剩余的蛋白质变性。碱性提取未降解的蛋白质，产率相当于物料中总蛋白质的三分之一，通过激活麦麸酶并结合奥斯本分级法，产率提高到62±8%。其余蛋白质以降解形式提取，总蛋白质含量的最大产率为91.6±1.6%。细麸和粗麸中的剩余物料的纤维含量平均相当于73±3%。当前的工作允许分离几种化合物，这使得麸皮原料能够转化为多种产品。
