Carmona-Gil Ana M, Sánchez Jorge, Maldonado-Estrada Juan
One Health and Veterinary Innovative Research and Development Group, Faculty of Agrarian Sciences, School of Veterinary Medicine, University of Antioquia, Medellin, Colombia.
Centro de Dermatología Veterinaria DermaVet, Medellin, Colombia.
Front Vet Sci. 2019 Dec 17;6:448. doi: 10.3389/fvets.2019.00448. eCollection 2019.
Skin prick-test is the first choice for the detection of type I hypersensitivity in human atopic dermatitis. Canine atopic dermatitis resembles several symptoms of the disease in the human counterpart. In canine atopic dermatitis, intradermal testing is the test of choice, and there are few reports on the use of skin prick test (SPT) in dogs. The purpose of this study was to evaluate SPT reactions in atopic dogs and a healthy control group to 11 environmental allergens. Eleven glycerinated allergens were applied on the left lateral thorax of nine atopic dogs and nine healthy dogs. The skin was pricked with a feather lancet and evaluated for the positive percutaneous reaction at 5, 10, 15, and 20 min after the application of the allergens. Data were analyzed by the Shapiro-Wilk test to test for normal distribution. Data that did not meet normality were analyzed by a one-sided Wilcoxon ranked sum test with a -value of 0.05. Six out of 9 atopic dogs tested positive for at least one of the allergens tested. None of the dogs in the control group showed a positive reaction to the allergens included in the test. , and exhibited the highest reaction rate among the group of atopic dogs. There was not a statistical difference in the histamine reaction (positive control) between both groups. In this set of dogs, the test exhibited a 100% specificity and a sensitivity of 66%. The use of skin prick-test in the detection of causative allergens of human atopic dermatitis has proved to be a sensitive and specific tool frequently used by human allergists. Due to the number of similarities in canine and human atopic dermatitis, this could be a valuable tool that needs intensive research in veterinary medicine. The published research so far correlates to the results obtained in this investigation. However, future studies evaluating the concordance between specific IgE antibody assays and SPT must be carried out simultaneously to validate the test.
皮肤点刺试验是检测人类特应性皮炎中Ⅰ型超敏反应的首选方法。犬特应性皮炎与人类该疾病的多种症状相似。在犬特应性皮炎中，皮内试验是首选检测方法，而关于犬皮肤点刺试验（SPT）的应用报道较少。本研究的目的是评估特应性犬和健康对照组对11种环境过敏原的SPT反应。将11种甘油化过敏原应用于9只特应性犬和9只健康犬的左胸外侧。用羽毛采血针针刺皮肤，并在应用过敏原后5、10、15和20分钟评估经皮阳性反应。数据通过Shapiro-Wilk检验分析以检验正态分布。不符合正态分布的数据通过单侧Wilcoxon秩和检验分析，α值为0.05。9只特应性犬中有6只对至少一种检测的过敏原呈阳性反应。对照组中没有一只犬对检测中的过敏原呈阳性反应。 、 和 在特应性犬组中表现出最高的反应率。两组之间组胺反应（阳性对照）没有统计学差异。在这组犬中，该检测表现出100%的特异性和66%的敏感性。皮肤点刺试验在检测人类特应性皮炎的致病过敏原方面已被证明是人类过敏症专家经常使用的一种敏感且特异的工具。由于犬和人类特应性皮炎有许多相似之处，这可能是一种有价值的工具，需要在兽医学中进行深入研究。迄今为止发表的研究与本调查获得的结果相关。然而，未来必须同时进行评估特异性IgE抗体检测和SPT之间一致性的研究，以验证该检测。
