Lin Jie, Jiang Jun, Zhou Ruifang, Li Xiaojie, Ye Jun
Department of Cardiology, Taizhou People's Hospital, Taizhou 225300, China.
Taizhou Polytechnic College, Taizhou 225300, China.
Open Med (Wars). 2019 Dec 26;15:1-7. doi: 10.1515/med-2020-0001. eCollection 2018.
Coronary artery disease (CAD) is one of the main causes of hospitalization worldwide and has high morbidity. MicroRNAs (miRNAs) play an important role in the pathogenesis of cardiovascular diseases. miR-451 is a special miRNA that is involved in many cancers' development. At present, there is no research about miR-451 in coronary heart disease. In this study, we aimed to identify the action mechanism of miR-451 in coronary heart disease and human umbilical vein endothelial cells (HUVECs). In this study, we found that miR-451 is up-regulated in the peripheral blood of patients with coronary heart disease. Moreover, TargetScan and dual-luciferase reporter gene assay results showed that VEGFA is a direct target gene of miR-451. C (CCK-8) and flow cytometry assay results showed that miR-451 mimic significantly inhibits cell proliferation and promotes apoptosis in HUVECs. Moreover, we found that the role of miR-451 in HUVECs is associated with the PI3K-Akt-mTOR pathway. Taken together, the data indicates that miR-451 might be a novel bio-marker for coronary heart disease.
冠状动脉疾病（CAD）是全球范围内住院治疗的主要原因之一，发病率很高。微小RNA（miRNA）在心血管疾病的发病机制中起重要作用。miR-451是一种特殊的miRNA，参与多种癌症的发展。目前，尚无关于miR-451在冠心病中的研究。在本研究中，我们旨在确定miR-451在冠心病和人脐静脉内皮细胞（HUVECs）中的作用机制。在本研究中，我们发现冠心病患者外周血中miR-451上调。此外，TargetScan和双荧光素酶报告基因检测结果表明，VEGFA是miR-451的直接靶基因。CCK-8和流式细胞术检测结果表明，miR-451模拟物显著抑制HUVECs细胞增殖并促进其凋亡。此外，我们发现miR-451在HUVECs中的作用与PI3K-Akt-mTOR信号通路有关。综上所述，数据表明miR-451可能是冠心病的一种新型生物标志物。
