Department of Chemistry and Technology of Polymers, Cracow University of Technology, 24 Warszawska Street, 31-155, Kraków, Poland.
Department of Chemistry and Technology of Polymers, Cracow University of Technology, 24 Warszawska Street, 31-155, Kraków, Poland.
Mater Sci Eng C Mater Biol Appl. 2020 Mar;108:110370. doi: 10.1016/j.msec.2019.110370. Epub 2019 Nov 2.
This work reports for the first time on a new class of flexible polyurethane foam hybrids (PUFs) synthesized with the use of less toxic aliphatic hexamethylene diisocyanate (HDI), which have been chemically modified by POSS moieties. The flexible polyurethane foam hybrids (PUFs) chemically modified by functionalized polyhedral oligomeric silsesquioxanes: octa(3-hydroxy-3-methylbutyldimethylsiloxy)POSS (OCTA-POSS) and 1,2-propanediolizo-butylPOSS (PHI-POSS), was obtained. The resulting foams, which contain 0 to 15 wt % POSS, were characterized in terms of their structure, morphology, density and compressive strength. The FT-IR results indicate the chemical incorporation of both OCTAPOSS and PHIPOSS into the polyurethane matrix. SEM-EDS analysis showed that both OCTAPOSS and PHIPOSS nanoparticles are distributed homogeneously in the foam structure; at 15 wt % load PHIPOSS characteristic "crosses" are formed. With the increase of PHIPOSS content in the matrix, the formation of agglomerates is observed, as revealed by WAXD spectra. The introduction of POSS compounds reduces the porosity of the polyurethane, with the number of pores increasing as the amount of modifier increases. Mechanical tests - compressive strength - show that the hardness of modified materials (5 wt % POSS) increases compared to the reference material. An incubation was carried out in a simulated physiological fluid (SBF) to pre-assess the bioactivity of the materials obtained. The obtained results confirmed the formation of a hydroxyapatite layer on the PUF-POSS surface. Cytotoxicity, cell cycle and apoptosis of osteoblast cells and fibroblasts were assessed in the presence of the PUF-POSS materials. Test materials have a cytotoxic effect on both established cell lines. PUF-PHIPOSS samples showed better biocompatibility than reference and PUF-OCTAPOSS samples, as they caused lower mortality of the examined cells.
这是首次报道一类新型的柔性聚氨酯泡沫杂化材料（PUFs），它们是使用毒性较低的脂肪族六亚甲基二异氰酸酯（HDI）合成的，并通过 POSS 基团进行了化学修饰。通过功能化的多面体低聚倍半硅氧烷（POSS），即八（3-羟基-3-甲基丁基二甲基硅氧基）POSS（OCTA-POSS）和 1,2-丙二醇异丁基 POSS（PHI-POSS）对柔性聚氨酯泡沫杂化材料（PUFs）进行了化学修饰。含有 0 至 15wt% POSS 的所得泡沫在结构、形态、密度和压缩强度方面进行了表征。FT-IR 结果表明，OCTAPOSS 和 PHI-POSS 均已化学结合到聚氨酯基质中。SEM-EDS 分析表明，OCTAPOSS 和 PHI-POSS 纳米粒子在泡沫结构中均匀分布；在 15wt%的负载下，形成 PHI-POSS 特有的“十字”。随着基质中 PHI-POSS 含量的增加，通过 WAXD 光谱观察到团聚体的形成。POSS 化合物的引入降低了聚氨酯的孔隙率，随着改性剂用量的增加，孔隙数量增加。力学测试 - 压缩强度 - 表明与参考材料相比，改性材料（5wt% POSS）的硬度增加。在模拟生理流体（SBF）中进行了孵育，以预先评估所获得材料的生物活性。获得的结果证实了在 PUF-POSS 表面形成了羟基磷灰石层。在存在 PUF-POSS 材料的情况下，评估了成骨细胞和纤维母细胞的细胞毒性、细胞周期和细胞凋亡。测试材料对两种已建立的细胞系均具有细胞毒性作用。与参考材料和 PUF-OCTAPOSS 样品相比，PUF-PHIPOSS 样品表现出更好的生物相容性，因为它们导致所检查细胞的死亡率较低。
