苯乙霉素，一种新型的埃弗霉素类抗生素复合物。II. 分离与结构测定。

Phenelfamycins, a novel complex of elfamycin-type antibiotics. II. Isolation and structure determination.

作者信息

Hochlowski J E, Buytendorp M H, Whittern D N, Buko A M, Chen R H, McAlpine J B

机构信息

Pharmaceutical Products Division, Abbott Laboratories, Abbott Park, Illinois 60064.

出版信息

J Antibiot (Tokyo). 1988 Oct;41(10):1300-15. doi: 10.7164/antibiotics.41.1300.

DOI:10.7164/antibiotics.41.1300

PMID:3192490

Abstract

A novel complex of elfamycin-type antibiotics has been isolated from submerged fermentation of either Streptomyces violaceoniger AB 999F-80 or Streptomyces violaceoniger AB 1047T-33. Antibiotics were extracted from the fermentation broth with ethyl acetate and from the mycelia with acetone. Purification of individual components was achieved by a combination of solvent partitions, Sephadex LH-20 exclusion, C18 bonded-phase silica gel adsorption, diol partition and liquid-liquid countercurrent chromatographies. Seven closely related components were separated and assigned structures 4, 11, 12, 13, 14, and 16 to phenelfamycins A to F respectively and structure 17 to unphenelfamycin. These structures were elucidated employing a variety of spectroscopic techniques, including extensive use of 1D and 2D NMR spectroscopy.

摘要

一种新型的埃弗霉素类抗生素复合物已从紫色链霉菌AB 999F - 80或紫色链霉菌AB 1047T - 33的深层发酵中分离得到。抗生素从发酵液中用乙酸乙酯萃取，从菌丝体中用丙酮萃取。通过溶剂分配、葡聚糖凝胶LH - 20排阻、C18键合相硅胶吸附、二醇分配和液 - 液逆流色谱等方法的组合实现了各个组分的纯化。分离出了七个密切相关的组分，分别将苯埃弗霉素A至F的结构指定为4、11、12、13、14和16，将非苯埃弗霉素的结构指定为17。这些结构是采用多种光谱技术阐明的，包括广泛使用一维和二维核磁共振光谱。