嗜热醋酸梭菌对镍的能量依赖性高亲和力转运。
Energy-dependent, high-affinity transport of nickel by the acetogen Clostridium thermoaceticum.
作者信息
Lundie L L, Yang H C, Heinonen J K, Dean S I, Drake H L
机构信息
Department of Biology, University of Mississippi, University 38677.
出版信息
J Bacteriol. 1988 Dec;170(12):5705-8. doi: 10.1128/jb.170.12.5705-5708.1988.
Abstract
The nickel transport system of Clostridium thermoaceticum was investigated with 63NiCl2 and an anaerobic microfiltration transport assay. Transport was optimal at pH 7 to pH 7.5 and 65 degrees C and decreased in the presence of metabolic uncouplers and inhibitors. Exogenous nickel was concentrated 3,000-fold over the apparent nickel concentration gradient during typical transport assays. Stored cellular energy appeared to provide a short-term energy source to power nickel transport, and starvation experiments demonstrated external energy source stimulation of nickel translocation. The apparent Km and Vmax for nickel transport by carbon monoxide-dependent chemolithotrophic cells approximated 3.2 microM Ni and 400 pmol of Ni transported per min per mg of cells (dry weight), respectively. Magnesium, calcium, cobalt, iron, manganese, and zinc did not inhibit the transport of nickel.
摘要
利用63NiCl2和厌氧微滤运输分析法对嗜热醋酸梭菌的镍转运系统进行了研究。转运在pH 7至pH 7.5以及65摄氏度时最为适宜，在存在代谢解偶联剂和抑制剂的情况下转运会减少。在典型的转运分析中，外源镍在表观镍浓度梯度上被浓缩了3000倍。储存的细胞能量似乎提供了一种短期能量来源来驱动镍的转运，饥饿实验表明外部能量源会刺激镍的转运。一氧化碳依赖的化能无机营养细胞转运镍的表观Km和Vmax分别约为3.2微摩尔镍和每分钟每毫克细胞（干重）转运400皮摩尔镍。镁、钙、钴、铁、锰和锌不会抑制镍的转运。
相似文献
1
Energy-dependent, high-affinity transport of nickel by the acetogen Clostridium thermoaceticum.
J Bacteriol. 1988 Dec;170(12):5705-8. doi: 10.1128/jb.170.12.5705-5708.1988.
2
Energy-dependent transport of nickel by Clostridium pasteurianum.
J Bacteriol. 1988 Jan;170(1):234-8. doi: 10.1128/jb.170.1.234-238.1988.
3
Nickel transport by the thermophilic acetogen Acetogenium kivui.
Appl Environ Microbiol. 1989 May;55(5):1078-81. doi: 10.1128/aem.55.5.1078-1081.1989.
4
Characterization of the H2- and CO-dependent chemolithotrophic potentials of the acetogens Clostridium thermoaceticum and Acetogenium kivui.
J Bacteriol. 1990 Aug;172(8):4464-71. doi: 10.1128/jb.172.8.4464-4471.1990.
5
Carbon monoxide-dependent chemolithotrophic growth of Clostridium thermoautotrophicum.
Appl Environ Microbiol. 1987 Aug;53(8):1902-6. doi: 10.1128/aem.53.8.1902-1906.1987.
6
Nitrate as a preferred electron sink for the acetogen Clostridium thermoaceticum.
J Bacteriol. 1993 Dec;175(24):8008-13. doi: 10.1128/jb.175.24.8008-8013.1993.
7
Transport and accumulation of nickel ions in the cyanobacterium Anabaena cylindrica.
Arch Biochem Biophys. 1986 Feb 1;244(2):470-7. doi: 10.1016/0003-9861(86)90615-6.
8
Development of a minimally defined medium for the acetogen Clostridium thermoaceticum.
J Bacteriol. 1984 Aug;159(2):700-3. doi: 10.1128/jb.159.2.700-703.1984.
9
Biotransformations of carboxylated aromatic compounds by the acetogen Clostridium thermoaceticum: generation of growth-supportive CO2 equivalents under CO2-limited conditions.
J Bacteriol. 1990 Jan;172(1):212-7. doi: 10.1128/jb.172.1.212-217.1990.
10
Nickel uptake in Bradyrhizobium japonicum.
J Bacteriol. 1987 Apr;169(4):1398-402. doi: 10.1128/jb.169.4.1398-1402.1987.
引用本文的文献
1
Expression Changes in Metal-Resistance Genes in Microbacterium liquefaciens Under Nickel and Vanadium Exposure.
Curr Microbiol. 2017 Jul;74(7):840-847. doi: 10.1007/s00284-017-1252-8. Epub 2017 Apr 27.
2
Oxalate- and Glyoxylate-Dependent Growth and Acetogenesis by Clostridium thermoaceticum.
Appl Environ Microbiol. 1993 Sep;59(9):3062-9. doi: 10.1128/aem.59.9.3062-3069.1993.
3
Ni(2+) transport and accumulation in Rhodospirillum rubrum.
J Bacteriol. 1999 Aug;181(15):4554-60. doi: 10.1128/JB.181.15.4554-4560.1999.
4
Precipitation of cadmium by Clostridium thermoaceticum.
Appl Environ Microbiol. 1993 Jan;59(1):7-14. doi: 10.1128/aem.59.1.7-14.1993.
5
Nickel transport by the thermophilic acetogen Acetogenium kivui.
Appl Environ Microbiol. 1989 May;55(5):1078-81. doi: 10.1128/aem.55.5.1078-1081.1989.
6
Characterization of the H2- and CO-dependent chemolithotrophic potentials of the acetogens Clostridium thermoaceticum and Acetogenium kivui.
J Bacteriol. 1990 Aug;172(8):4464-71. doi: 10.1128/jb.172.8.4464-4471.1990.
7
Differential effects of sodium on hydrogen- and glucose-dependent growth of the acetogenic bacterium Acetogenium kivui.
Appl Environ Microbiol. 1990 Jan;56(1):81-6. doi: 10.1128/aem.56.1.81-86.1990.
8
Nickel accumulation and storage in Bradyrhizobium japonicum.
Appl Environ Microbiol. 1990 Jun;56(6):1905-11. doi: 10.1128/aem.56.6.1905-1911.1990.
9
Expression of an aromatic-dependent decarboxylase which provides growth-essential CO2 equivalents for the acetogenic (Wood) pathway of Clostridium thermoaceticum.
J Bacteriol. 1990 Oct;172(10):5901-7. doi: 10.1128/jb.172.10.5901-5907.1990.
10
Biotransformations of carboxylated aromatic compounds by the acetogen Clostridium thermoaceticum: generation of growth-supportive CO2 equivalents under CO2-limited conditions.
J Bacteriol. 1990 Jan;172(1):212-7. doi: 10.1128/jb.172.1.212-217.1990.
本文引用的文献
1
Methanophosphagen: Unique cyclic pyrophosphate isolated from Methanobacterium thermoautotrophicum.
Proc Natl Acad Sci U S A. 1983 Sep;80(17):5217-21. doi: 10.1073/pnas.80.17.5217.
2
Membrane H Conductance of Clostridium thermoaceticum and Clostridium acetobutylicum: Evidence for Electrogenic Na/H Antiport in Clostridium thermoaceticum.
Appl Environ Microbiol. 1987 Apr;53(4):782-6. doi: 10.1128/aem.53.4.782-786.1987.
3
Uncoupling by Acetic Acid Limits Growth of and Acetogenesis by Clostridium thermoaceticum.
Appl Environ Microbiol. 1984 Dec;48(6):1134-9. doi: 10.1128/aem.48.6.1134-1139.1984.
4
Nickel transport in Methanobacterium bryantii.
J Bacteriol. 1982 Sep;151(3):1195-203. doi: 10.1128/jb.151.3.1195-1203.1982.
5
Spontaneous protoplast formation in Methanobacterium bryantii.
J Bacteriol. 1982 Jan;149(1):346-53. doi: 10.1128/jb.149.1.346-353.1982.
6
Effect of chelating agents on hydrogenase in Azotobacter chroococcum. Evidence that nickel is required for hydrogenase synthesis.
Biochem J. 1982 Apr 15;204(1):339-44. doi: 10.1042/bj2040339.
7
Occurrence of nickel in carbon monoxide dehydrogenase from Clostridium pasteurianum and Clostridium thermoaceticum.
J Bacteriol. 1982 Feb;149(2):561-6. doi: 10.1128/jb.149.2.561-566.1982.
8
A novel diphospho-P,P'-diester from Methanobacterium thermoautotrophicum.
J Biol Chem. 1983 Sep 25;258(18):10835-8.
9
Development of a minimally defined medium for the acetogen Clostridium thermoaceticum.
J Bacteriol. 1984 Aug;159(2):700-3. doi: 10.1128/jb.159.2.700-703.1984.
10
Peptostreptococcus productus strain that grows rapidly with CO as the energy source.
Appl Environ Microbiol. 1984 May;47(5):961-4. doi: 10.1128/aem.47.5.961-964.1984.
Zotero 插件