Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
Proc Natl Acad Sci U S A. 1983 Sep;80(17):5217-21. doi: 10.1073/pnas.80.17.5217.
A unique cyclic pyrophosphate compound has been detected at 10-12 mM intracellular concentration in Methanobacterium thermoautotrophicum by in vivo(31)P NMR. This compound has been extracted from cells and purified by anion-exchange chromatography. Studies with (1)H, (13)C, and (31)P NMR and fast-atom-bombardment mass spectrometry have identified it as 2,3-cyclopyrophosphoglycerate, an intramolecularly cyclized pyrophosphate of 2,3-diphosphoglycerate. Chemical degradation to 2,3-diphosphoglycerate and synthesis by dicyclohexylcarbodiimide coupling of 2,3-diphosphoglycerate are consistent with this identification. It is suggested that this compound serves as a primary phosphagen in methanogens.
在产甲烷菌 Methanobacterium thermoautotrophicum 中,通过体内(31)P NMR 检测到细胞内浓度为 10-12mM 的独特环状焦磷酸化合物。该化合物已从细胞中提取出来,并通过阴离子交换色谱法进行了纯化。通过(1)H、(13)C 和(31)P NMR 以及快原子轰击质谱研究,将其鉴定为 2,3-环焦磷酸甘油酸,这是 2,3-二磷酸甘油酸的分子内环状焦磷酸。化学降解为 2,3-二磷酸甘油酸和二环己基碳化二亚胺偶联 2,3-二磷酸甘油酸的合成与该鉴定一致。有人认为这种化合物是甲烷菌中主要的磷酸原。