酵母双链RNA与核DNA之间的同源性。
Homology between double-stranded RNA and nuclear DNA of yeast.
作者信息
Vodkin M
出版信息
J Virol. 1977 Feb;21(2):516-21. doi: 10.1128/JVI.21.2.516-521.1977.
Abstract
The relationship between mycoviral double-stranded (ds) RNA and host cell DNA was investigated. Radiolabeled ds RNA was denatured and reannealed in the presence and absence of denatured DNA. RNA from killer strains of the yeast Saccharomyces cerevisiae and from nonkiller derivatives was utilized. The above-mentioned strains, as well as one that lacks all ds RNA, were sources for extracted DNA. Net hybridization of ds RNA to DNA occurred regardless of the strains from which the respective nucleic acids were prepared.
摘要
研究了真菌病毒双链（ds）RNA与宿主细胞DNA之间的关系。将放射性标记的ds RNA在有和没有变性DNA存在的情况下进行变性和复性。使用了来自酿酒酵母杀伤菌株和非杀伤衍生物的RNA。上述菌株以及一种缺乏所有ds RNA的菌株是提取DNA的来源。无论制备各自核酸的菌株如何，ds RNA与DNA之间都会发生净杂交。
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