我们是否应该更加谨慎？重组的一个警示故事。
Should we be more cre-tical? A cautionary tale of recombination.
机构信息
Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, UK.
出版信息
Immunology. 2020 Feb;159(2):131-132. doi: 10.1111/imm.13170.
Abstract
The cre-loxP system has been revolutionary in the field of immunology. The technology enables genetic deletion in mice with unprecedented precision. It is therefore now widely used to investigate gene functions in animal models of disease, and in fundamental studies of the immune response. This widespread adoption of cre-loxP technology has allowed a thorough investigation of its strengths and weaknesses. Here we highlight an important paper which not only describes potential problems with the commonly-used screening procedures used when identifying offspring of the correct genotype, but also describes how this screening can be improved to ensure that the right animals are produced.
摘要
Cre-loxP 系统在免疫学领域具有革命性意义。该技术能够以前所未有的精度在小鼠中进行基因缺失。因此，它现在被广泛用于研究疾病动物模型中的基因功能，以及免疫反应的基础研究。Cre-loxP 技术的广泛采用使得对其优缺点进行了彻底的研究。在这里，我们重点介绍一篇重要的论文，该论文不仅描述了在鉴定正确基因型后代时常用的筛选程序中存在的潜在问题，还描述了如何改进这种筛选，以确保生产出正确的动物。
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